{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:02:59Z","timestamp":1790139779574,"version":"4.0.1"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:00:00Z","timestamp":1768435200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPlusChem"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    The effect of CuSO\n                    <jats:sub>4<\/jats:sub>\n                    on the stability of an immobilized laccase during the oxidation of HMF to FFCA was determined in a batch bioreactor and in a triphasic flow bioreactor. The enzyme was immobilized on a support functionalized with an epoxide and glyoxyl group; the latter achieved the highest immobilization yield by activity (87.5%). In batch oxidation of HMF catalyzed by laccase immobilized on a support with glyoxyl groups reached the highest conversion percentages (100%). In the biocatalyst stability, the addition of 20\u2009mM CuSO\n                    <jats:sub>4<\/jats:sub>\n                    allowed achieving 50% HMF conversion at the end of the third cycle. In the absence of CuSO\n                    <jats:sub>4<\/jats:sub>\n                    , the biocatalyst was unable to catalyze the reaction. The oxidation in packed\u2010bed flow bioreactor resulted in the production of FFCA only. With CuSO\n                    <jats:sub>4<\/jats:sub>\n                    , the stability of the biocatalyst was maintained for 48\u2009h, with a FFCA yield of 95%. The UM values (0.2\u20135\u2009cm\/min) demonstrated that above an air flow rate of 0.1\u2009mL\/min, there are no mass transfer problems affecting the reaction yield. The space\u2010time yield of FFCA (STY FFCA) (1.54 (mM\/min)) was higher for the continuous system in the presence of CuSO\n                    <jats:sub>4<\/jats:sub>\n                    . Overall, this system provides a promising and scalable platform for sustainable industrial biocatalysis.\n                  <\/jats:p>","DOI":"10.1002\/cplu.202500650","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T23:17:25Z","timestamp":1768519045000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Three\u2010Phasic Flow Bioreactor for Obtaining 5\u2010Formyl\u20102\u2010Furancarboxylic Acid Catalyzed by an Immobilized Laccase Using Copper Sulfate as a Stabilizing Agent"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-133X","authenticated-orcid":false,"given":"Nadia","family":"Guajardo","sequence":"first","affiliation":[{"name":"Departamento de Ingenier\u00eda Qu\u00edmica y Bioprocesos Escuela de Ingenier\u00eda Pontificia Universidad Cat\u00f3lica de Chile  Santiago 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