{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:59:23Z","timestamp":1790319563334,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["DST\u2010INSPIRE\u2010SRF"],"award-info":[{"award-number":["DST\u2010INSPIRE\u2010SRF"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007853","name":"Central ElectroChemical Research Institute","doi-asserted-by":"publisher","award":["21(1108)\u201020\/EMR\u2010II"],"award-info":[{"award-number":["21(1108)\u201020\/EMR\u2010II"]}],"id":[{"id":"10.13039\/501100007853","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["GAP 11\/23"],"award-info":[{"award-number":["GAP 11\/23"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006593","name":"Board of Research in Nuclear Sciences","doi-asserted-by":"publisher","award":["GAP 06\/20"],"award-info":[{"award-number":["GAP 06\/20"]}],"id":[{"id":"10.13039\/501100006593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPlusChem"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:p>\n                    A significant challenge in creating enzyme\u2010based electrochemical biosensors is the immobilization of enzymes onto the electrode surface while retaining their activity. High surface area, tunable porosity, biocompatibility, and stability of metal\u2010organic frameworks (MOFs) make them a suitable matrix for the immobilization of enzymes. Besides acting as an effective immobilization platform, MOFs exhibit interesting enzyme\u2010mimicking capabilities as well. In this study, we demonstrate dual functionality of copper\u2010incorporated zeolitic imidazolate framework\u20108 (Cu@ZIF\u20108) both as a peroxidase mimic and as an efficient immobilization matrix for the enzyme,\n                    <jats:italic>cholesterol oxidase<\/jats:italic>\n                    (ChO\n                    <jats:sub>\n                      <jats:italic>x<\/jats:italic>\n                    <\/jats:sub>\n                    ), enabling the electrochemical sensing of cholesterol. ChO\n                    <jats:sub>\n                      <jats:italic>x<\/jats:italic>\n                    <\/jats:sub>\n                    enzyme is immobilized onto Cu@ZIF\u20108 using biomimetic mineralization strategy. Enhanced sensitivity and lowered Michaelis\u2013Menten constant values are observed for enzyme\u2010immobilized system compared to the nonimmobilized system, indicating the stronger substrate affinity of the developed sensing platform. This suggests that Cu@ZIF\u20108 acts as a scaffold for the enzyme immobilization and improves the enzyme stability and reaction kinetics. Moreover, this strategy also avoids the complications of using multiple enzymes for sensing purposes. This dual functionality of MOFs enhances the stability and selectivity of the biosensor while eliminating the use of multiple enzymes and thus paves the way for the fabrication of cost\u2010effective and robust next\u2010generation biosensors.\n                  <\/jats:p>","DOI":"10.1002\/cplu.202600008","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T22:10:30Z","timestamp":1789596630000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bioenzymatic Approach to Cholesterol Detection Using Cholesterol Oxidase\u2010Immobilized Copper\u2010Incorporated Zeolitic Imidazolate Framework\u20108"],"prefix":"10.1002","volume":"91","author":[{"given":"Aswathi","family":"Mechoor","sequence":"first","affiliation":[{"name":"Electrodics and Electrocatalysis (EEC) Division CSIR \u2013 Central Electrochemical Research Institute (CSIR \u2013 CECRI)  Karaikudi Tamil Nadu India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"},{"name":"School of Life and Environmental Sciences Deakin University  Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na","family":"Kong","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences Deakin University  Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheela","family":"Berchmans","sequence":"additional","affiliation":[{"name":"Electrodics and Electrocatalysis (EEC) Division CSIR \u2013 Central Electrochemical Research Institute (CSIR \u2013 CECRI)  Karaikudi Tamil Nadu India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenrong","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences Deakin University  Geelong Victoria 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