{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,25]],"date-time":"2026-10-25T22:33:23Z","timestamp":1792967603871,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T00:00:00Z","timestamp":1792627200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemPlusChem"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:p>\n                    The quantification of albumin levels in biological samples is a critical biomarker for diagnosing and monitoring various pathological conditions. This study introduced a vitamin B\n                    <jats:sub>6<\/jats:sub>\n                    cofactor derived Schiff base (E)\u2010(4\u2010(((2\u2010(1H\u2010benzo[d]imidazol\u20102\u2010yl)phenyl)imino)methyl)\u20105\u2010hydroxy\u20106\u2010methylpyridin\u20103\u2010yl)methyl dihydrogen phosphate (BMLP) via the condensation of pyridoxal 5\u2032\u2010phosphate with 2\u2010(1H\u2010benzo[d]imidazol\u20102\u2010yl)aniline. Schiff base BMLP formed a complex with Fe\n                    <jats:sup>3+<\/jats:sup>\n                    in 2:1 binding ratio and redshifted its fluorescence emission from 417 to 474\u2009nm, accompanied by slight quenching at 417\u2009nm and fluorescence enhancement at 474\u2009nm. The in situ formed BMLP\u2500Fe\n                    <jats:sup>3+<\/jats:sup>\n                    complex was employed for the ratiometric fluorescence sensing of albumins, that is, human serum albumin (HSA) and bovine serum albumin (BSA). With the addition of HSA and BSA, the fluorescence emission of BMLP\u2500Fe\n                    <jats:sup>3+<\/jats:sup>\n                    complex was quenched at 474\u2009nm and enhanced at 417\u2009nm. The selective ratiometric response from the BMLP\u2500Fe\n                    <jats:sup>3+<\/jats:sup>\n                    complex can be employed to detect HSA and BSA down to 3.8 and 3.6\u2009 \u00b5M, respectively. The developed fluorescence\u2010based sensing approach was applied for quantifying HSA and BSA in real biological matrices, including milk and urine, with satisfactory recovery rates.\n                  <\/jats:p>","DOI":"10.1002\/cplu.70275","type":"journal-article","created":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:48:27Z","timestamp":1792723707000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ratiometric Fluorescence Sensing of Human\/Bovine Serum Albumin Using In Situ Formed Fe\n                    <sup>3+<\/sup>\n                    Complex"],"prefix":"10.1002","volume":"91","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1339-4560","authenticated-orcid":false,"given":"Kanishk","family":"Bhardwaj","sequence":"first","affiliation":[{"name":"Department of Chemistry Sardar Vallabhbhai National Institute Technology  Surat Gujarat 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