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Infections are typically initiated through the consumption of cyst\u2010contaminated water or food. <jats:italic>Giardia<\/jats:italic> was first axenized in the 1970s and can be readily maintained in a laboratory setting. Additionally, <jats:italic>Giardia<\/jats:italic> is one of the few protozoans that can be induced to complete its complete lifecycle using laboratory methods. In this article, we outline protocols to maintain <jats:italic>Giardia<\/jats:italic> and induce passage through its lifecycle. We also provide protocols for infecting and quantifying parasites in an animal infection model. \u00a9 2020 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: In vitro maintenance and growth of <jats:italic>Giardia<\/jats:italic> trophozoites<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: In vitro encystation of <jats:italic>Giardia<\/jats:italic> cysts<\/jats:p><jats:p><jats:bold>Basic Protocol 3<\/jats:bold>: In vivo infections using <jats:italic>Giardia<\/jats:italic> trophozoites<\/jats:p>","DOI":"10.1002\/cpmc.102","type":"journal-article","created":{"date-parts":[[2020,6,9]],"date-time":"2020-06-09T12:53:23Z","timestamp":1591707203000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["<i>Giardia lamblia<\/i>: Laboratory Maintenance, Lifecycle Induction, and Infection of Murine Models"],"prefix":"10.1002","volume":"57","author":[{"given":"Marc 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