{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T02:07:08Z","timestamp":1768615628000,"version":"3.49.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T00:00:00Z","timestamp":1598572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000270","name":"Natural Environment Research Council","doi-asserted-by":"publisher","award":["NE\/P001165\/1"],"award-info":[{"award-number":["NE\/P001165\/1"]}],"id":[{"id":"10.13039\/501100000270","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/R015600\/1"],"award-info":[{"award-number":["MR\/R015600\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The global emergence of azole resistance in <jats:italic>Aspergillus fumigatus<\/jats:italic> is resulting in health and food security concerns. Rapid diagnostics and environmental surveillance methods are key to understanding the distribution and prevalence of azole resistance. However, such methods are often associated with high costs and are not always applicable to laboratories based in the least\u2010developed countries. Here, we present and validate a low\u2010cost screening protocol that can be used to differentiate between azole\u2010susceptible \u201cwild\u2010type\u201d and azole\u2010resistant <jats:italic>A. fumigatus<\/jats:italic> isolates. \u00a9 2020 The Authors.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Preparation of Tebucheck multi\u2010well plates<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: Inoculation of Tebucheck multi\u2010well plates<\/jats:p>","DOI":"10.1002\/cpmc.112","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T22:13:54Z","timestamp":1598652834000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Low\u2010Cost Tebuconazole\u2010Based Screening Test for Azole\u2010Resistant <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1002","volume":"58","author":[{"given":"Amelie P.","family":"Brackin","sequence":"first","affiliation":[{"name":"MRC Centre for Global Infectious Disease Analysis, Department of Infectious Disease Epidemiology Imperial College London  London United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer M. 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