{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T05:54:46Z","timestamp":1790661286267,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T00:00:00Z","timestamp":1600992000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Candida albicans<\/jats:italic> is an opportunistic fungal pathogen and a model organism to study fungal pathogenesis. It exists as a harmless commensal organism and member of the healthy human microbiome, but can cause life\u2010threatening mucosal and systemic infections. A model host to study <jats:italic>C.\u00a0albicans<\/jats:italic> infection and pathogenesis is the nematode <jats:italic>Caenorhabditis elegans<\/jats:italic>. <jats:italic>C.\u00a0elegans<\/jats:italic> is frequently used as a model host to study microbial\u2010host interactions because it can be infected by many human pathogens and there are also close morphological resemblances between the intestinal cells of <jats:italic>C.\u00a0elegans<\/jats:italic> and mammals, where <jats:italic>C.\u00a0albicans<\/jats:italic> infections can occur. This article outlines a detailed methodology for exploiting <jats:italic>C.\u00a0elegans<\/jats:italic> as a host to study <jats:italic>C.\u00a0albicans<\/jats:italic> infection, including a <jats:italic>C.\u00a0elegans<\/jats:italic> egg preparation protocol and an agar\u2010based <jats:italic>C.\u00a0elegans<\/jats:italic> killing protocol to monitor fungal virulence. These protocols can additionally be used to study <jats:italic>C.\u00a0albicans<\/jats:italic> genetic mutants in order to further our understanding of the genes involved in pathogenesis and virulence in <jats:italic>C.\u00a0albicans<\/jats:italic> and the mechanisms of host\u2010microbe interactions. \u00a9 2020 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol 1<\/jats:bold>: Preparation of <jats:italic>Caenorhabditis elegans<\/jats:italic> eggs<\/jats:p><jats:p><jats:bold>Support Protocol 1<\/jats:bold>: Freezing and recovering <jats:italic>Caenorhabditis elegans<\/jats:italic><\/jats:p><jats:p><jats:bold>Support Protocol 2<\/jats:bold>: Making superfood agar and OP50 plates<\/jats:p><jats:p><jats:bold>Basic Protocol 2<\/jats:bold>: <jats:italic>Caenorhabditis elegans<\/jats:italic>\/<jats:italic>Candida albicans<\/jats:italic> agar killing assay<\/jats:p><jats:p><jats:bold>Support Protocol 3<\/jats:bold>: Constructing a worm pick<\/jats:p>","DOI":"10.1002\/cpmc.114","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T14:26:45Z","timestamp":1601044005000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A Simple Nematode Infection Model for Studying <i>Candida albicans<\/i> Pathogenesis"],"prefix":"10.1002","volume":"59","author":[{"given":"Grace H.","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sierra","family":"Rosiana","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cellular Biology University of Guelph  Guelph Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia V.","family":"Kirienko","sequence":"additional","affiliation":[{"name":"Department of BioSciences Rice University 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