{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:44:56Z","timestamp":1753868696646,"version":"3.41.2"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T00:00:00Z","timestamp":1602201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Giardia<\/jats:italic> is an enteric protozoan parasite that causes gastroenteritis in all classes of vertebrates. It is ranked among the leading causes of death in children under 5 years of age. Giardiasis affects approximately 280 million people worldwide annually, a situation exacerbated by the low availability of effective treatments and the lack of a vaccine. In addition, the parasite is difficult to manipulate in in vitro environments, which hampers the development of effective disease management strategies. This article highlights the development of a method for the purification of viable <jats:italic>Giardia<\/jats:italic> cysts from fecal samples, verified by a trypan blue dye exclusion test. This protocol produces a 10\u2010fold increase in yield over current methods. By combining sucrose flotation with gated filtration, the protocol significantly reduces the amount of debris in the purified cysts suspension. Cyst viability is verified by a trypan blue dye exclusion test. The ability to purify large quantities of <jats:italic>Giardia<\/jats:italic> from fecal samples could advance the development of effective treatments to target this worldwide prevalent parasite. \u00a9 2020 Wiley Periodicals LLC.<\/jats:p><jats:p><jats:bold>Basic Protocol<\/jats:bold>: Purification of <jats:italic>Giardia<\/jats:italic> cysts from fecal samples<\/jats:p><jats:p><jats:bold>Support Protocol<\/jats:bold>: Cyst viability test<\/jats:p>","DOI":"10.1002\/cpmc.117","type":"journal-article","created":{"date-parts":[[2020,10,9]],"date-time":"2020-10-09T12:32:39Z","timestamp":1602246759000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["High\u2010Yield Purification of <i>Giardia intestinalis<\/i> Cysts from Fecal Samples"],"prefix":"10.1002","volume":"59","author":[{"given":"Paul","family":"Ogbuigwe","sequence":"first","affiliation":[{"name":"Molecular Epidemiology and Public Health Laboratory, Hopkirk Research Institute Massey University  Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony B.","family":"Pita","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology and Public Health Laboratory, Hopkirk Research Institute Massey University  Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthew A.","family":"Knox","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology and Public Health Laboratory, Hopkirk Research Institute Massey University  Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niluka","family":"Velathanthiri","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology and Public Health Laboratory, Hopkirk Research Institute Massey University  Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David T. S.","family":"Hayman","sequence":"additional","affiliation":[{"name":"Molecular Epidemiology and Public Health Laboratory, Hopkirk Research Institute Massey University  Palmerston North New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,10,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2018.01.011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.5897\/AJB11.391"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0143-x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(79)90080-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc01a01s13"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007250"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-008-0968-6"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2017.01.036"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(89)90185-9"},{"key":"e_1_2_7_11_1","first-page":"3","article-title":"Giardia lamblia infection: Review of current diagnostic strategies","volume":"12","author":"Hooshyar H.","year":"2019","journal-title":"Gastroenterology and Hepatology from Bed to Bench"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1080\/10643388809388341"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02931953"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002512"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","unstructured":"Luj\u00e1n H. 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