{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T07:10:26Z","timestamp":1792480226259,"version":"4.5.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,2,13]],"date-time":"2018-02-13T00:00:00Z","timestamp":1518480000000},"content-version":"vor","delay-in-days":561,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Natural infections of <jats:italic>Vaccinia virus<\/jats:italic> (VACV)\u2014the prototype species of the <jats:italic>Orthopoxvirus<\/jats:italic> genus, from the family <jats:italic>Poxviridae<\/jats:italic> and subfamily <jats:italic>Chordopoxvirinae<\/jats:italic>\u2014cause an occupational emergent zoonotic disease that is primarily associated with the handling of infected dairy cattle. In humans, VACV infection is characterized by skin lesions, primarily on the hands, and accompanied by systemic symptoms such as fever, myalgia, headache, and lymphadenopathy. The diagnosis of VACV is usually performed according to the methods described for other orthopoxviruses. This unit describes the methods utilized to obtain clinical samples, the serological and molecular techniques used for diagnosis, and the isolation methods and techniques used for molecular and biological characterization of the viruses. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.13","type":"journal-article","created":{"date-parts":[[2016,8,12]],"date-time":"2016-08-12T18:34:30Z","timestamp":1471026870000},"source":"Crossref","is-referenced-by-count":16,"title":["Natural <i>Vaccinia Virus<\/i> Infection: Diagnosis, Isolation, and Characterization"],"prefix":"10.1002","volume":"42","author":[{"given":"Erna","family":"Geessien Kroon","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f4natas","family":"Santos Abrah\u00e3o","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giliane","family":"de Souza Trindade","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziele","family":"Pereira Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Moreira Franco Luiz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galileu","family":"Barbosa Costa","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Teixeira Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Silva Calixto","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danilo Bretas","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Faculdade de Medicina de Diamantina, Universidade Federal dos Vales do Jequitinhonha e Mucuri  Diamantina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bet\u00e2nia Paiva","family":"Drumond","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de V\u00edrus, Departamento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais  Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,13]]},"reference":[{"key":"e_1_2_19_2_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21617"},{"key":"e_1_2_19_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"key":"e_1_2_19_2_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0050413"},{"key":"e_1_2_19_2_4_1","first-page":"104","volume":"24","author":"Campos M.A.S.","year":"1993","journal-title":"Braz. 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