{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T06:17:53Z","timestamp":1789712273079,"version":"4.0.1"},"reference-count":6,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T00:00:00Z","timestamp":1479427200000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:italic>Blastocystis<\/jats:italic> is an intestinal parasite that is very easily isolated in culture from fresh stool samples. In fact, the parasite grows so readily in culture that short\u2010term in vitro culture is sometimes used as a diagnostic tool in the absence of DNA\u2010based methods. While axenizing <jats:italic>Blastocystis<\/jats:italic> cultures remains a significant challenge, the parasite can be propagated for several months in the presence of metabolically active bacteria (xenic culture). Hence, culture can be used for maintaining live <jats:italic>Blastocystis<\/jats:italic> strain libraries. This enables the production of a stable resource of reference material, which for instance can be used for DNA\u2010based assays and research. <jats:italic>Blastocystis<\/jats:italic> isolates can also be cryopreserved with a view to reestablishing them in culture. Here, we provide protocols for xenic in vitro culture and cryopreservation of <jats:italic>Blastocystis<\/jats:italic>. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.18","type":"journal-article","created":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T16:42:54Z","timestamp":1479487374000},"source":"Crossref","is-referenced-by-count":25,"title":["<i>Blastocystis<\/i>: Isolation, Xenic Cultivation, and Cryopreservation"],"prefix":"10.1002","volume":"43","author":[{"given":"C. Graham","family":"Clark","sequence":"first","affiliation":[{"name":"Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine  London United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. Rune","family":"Stensvold","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection, Statens Serum Institut  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,18]]},"reference":[{"key":"e_1_2_8_1_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.329-341.2002"},{"key":"e_1_2_8_1_3_1","doi-asserted-by":"publisher","DOI":"10.2307\/3281016"},{"key":"e_1_2_8_1_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1946.11685270"},{"key":"e_1_2_8_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(68)90170-3"},{"key":"e_1_2_8_1_6_1","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.2001.11813705"},{"key":"e_1_2_8_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.06.003"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.18","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T09:04:39Z","timestamp":1692954279000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.18"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/cpmc.18"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.18","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11]]}}}