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With the increased interest in ZIKV, protocols must be established to facilitate proper research. Here we describe the laboratory techniques required to quantify, propagate, and store ZIVK. We also review the proper safety protocol for the handling of ZIKV, which is classified as a Biosafety Level 2 pathogen by the United States Centers for Disease Control and Prevention. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.19","type":"journal-article","created":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T16:42:54Z","timestamp":1479487374000},"source":"Crossref","is-referenced-by-count":29,"title":["Zika Virus: Quantification, Propagation, Detection, and Storage"],"prefix":"10.1002","volume":"43","author":[{"given":"Darrell S.","family":"Agbulos","sequence":"first","affiliation":[{"name":"Centre for Biotechnology, Brock University  St. Catharines Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa","family":"Barelli","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology, Brock University  St. Catharines Ontario Canada"},{"name":"Entomogen Incorporated  St. Catharines Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bryan V.","family":"Giordano","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology, Brock University  St. Catharines Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona F.","family":"Hunter","sequence":"additional","affiliation":[{"name":"Centre for Biotechnology, Brock University  St. Catharines Ontario Canada"},{"name":"Entomogen Incorporated  St. Catharines Ontario Canada"},{"name":"Department of Biological Sciences, Brock University  St. Catharines Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,13]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"227","article-title":"Investigation surrounding a fatal case of yellow fever in C\u00f4te d'Ivoire in 1999","volume":"94","author":"Akoua\u2010Koffi C.","year":"2001","journal-title":"Bull. 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