{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:06:05Z","timestamp":1790787965754,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T00:00:00Z","timestamp":1479427200000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Anaerobic microorganisms play a central role in several environmental processes and regulate global biogeochemical cycling of nutrients and minerals. Many anaerobic microorganisms are important for the production of bioenergy and biofuels. However, the major hurdle in studying anaerobic microorganisms in the laboratory is the requirement for sophisticated and expensive gassing stations and glove boxes to create and maintain the anaerobic environment. This appendix presents a simple design for a gassing station that can be used readily by an inexperienced investigator for cultivation of anaerobic microorganisms. In addition, this appendix also details the low\u2010cost assembly of bioelectrochemical systems and outlines a simplified procedure for cultivating and analyzing bacterial cell cultures and biofilms that produce electric current, using\n                    <jats:italic>Geobacter sulfurreducens<\/jats:italic>\n                    as a model organism. \u00a9 2016 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cpmc.20","type":"journal-article","created":{"date-parts":[[2016,11,18]],"date-time":"2016-11-18T11:42:54Z","timestamp":1479469374000},"source":"Crossref","is-referenced-by-count":13,"title":["A Simple and Low\u2010Cost Procedure for Growing\n                    <i>Geobacter sulfurreducens<\/i>\n                    Cell Cultures and Biofilms in Bioelectrochemical Systems"],"prefix":"10.1002","volume":"43","author":[{"given":"J. Patrick","family":"O'Brien","sequence":"first","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry, Microbial Sciences Institute, Yale University  West Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikhil S.","family":"Malvankar","sequence":"additional","affiliation":[{"name":"Department of Molecular Biophysics and Biochemistry, Microbial Sciences Institute, Yale University  West Haven Connecticut"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,11,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119097426.ch1"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc01a01s13"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.4155\/bfs.10.25"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1217412"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-387661-4.00004-5"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2013.02.012"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.201100733"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2013.12.003"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2011.119"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2ee03388g"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01803-12"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.201100865"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2ee22330a"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119097426.ch7"},{"key":"e_1_2_12_16_1","volume-title":"Bioelectrochemical Systems: From Extracellular Electron Transfer to Biotechnological Application","author":"Rabaey K.","year":"2009"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781119097426.ch2"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mca04fs12"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201601112"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00210-13"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.20","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T05:04:35Z","timestamp":1692939875000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.20"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,11]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,11]]}},"alternative-id":["10.1002\/cpmc.20"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.20","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,11]]}}}