{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,26]],"date-time":"2023-12-26T19:51:49Z","timestamp":1703620309560},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T00:00:00Z","timestamp":1486339200000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:italic>Cryptosporidium<\/jats:italic> spp. are obligate protozoan parasites of the gastrointestinal tract of vertebrates, including humans. In the majority of human cases, the diarrheal disease cryptosporidiosis is caused by either the human\u2010adapted species <jats:italic>Cryptosporidium hominis<\/jats:italic> or the zoonotic <jats:italic>Cryptosporidium parvum<\/jats:italic> \u2018bovine genotype\u2019 (also known as <jats:italic>Cryptosporidium pestis<\/jats:italic>). The infectious stage, environmentally resilient <jats:italic>Cryptosporidium<\/jats:italic> oocysts, are shed by the infected host. <jats:italic>Cryptosporidium<\/jats:italic> parasites are transmitted by the fecal\u2010oral route and are one of the major water\u2010borne pathogens. The cryptic nature of the microscopic <jats:italic>Cryptosporidium<\/jats:italic> oocysts coupled with the existence of several host\u2010adapted and zoonotic species requires molecular tools to identify <jats:italic>Cryptosporidium<\/jats:italic> spp. in either fecal or environmental samples. This unit describes methods for <jats:italic>Cryptosporidium<\/jats:italic> identification and typing using genotyping based on nuclear loci. We also provide a protocol for morphological confirmation of <jats:italic>Cryptosporidium<\/jats:italic> oocysts based on antibody labeling of the <jats:italic>Cryptosporidium<\/jats:italic> oocyst wall and a protocol for purification of oocysts from fecal material. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.24","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T19:47:37Z","timestamp":1486410457000},"source":"Crossref","is-referenced-by-count":6,"title":["<i>Cryptosporidium<\/i>: Identification and Genetic Typing"],"prefix":"10.1002","volume":"44","author":[{"given":"Jan","family":"\u0160lapeta","sequence":"first","affiliation":[{"name":"Sydney School of Veterinary Science and School of Life and Environmental Sciences, Faculty of Science, University of Sydney  New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"e_1_2_7_3_1","unstructured":"Ausubel F.M. 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