{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:42:47Z","timestamp":1792806167295,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T00:00:00Z","timestamp":1494892800000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The protozoan parasite <jats:italic>Toxoplasma gondii<\/jats:italic> is capable of infecting all warm\u2010blooded animals and humans. Infectious, transmissible forms of the parasite include oocysts produced by the sexual cycle within the definitive feline host and tissue cysts that form <jats:italic>Toxoplasma<\/jats:italic> in the central nervous system and muscle during the asexual cycle within all chronically infected warm\u2010blooded hosts. These tissue cysts are populated with slow\u2010growing bradyzoites, which until recently have been thought to be dormant entities in the context of immune sufficiency. Reactivation to active growth during immune suppression is of critical clinical importance. However, little is known about tissue cysts or the bradyzoites they house, as the diversity of tissue cysts cannot be replicated in cell culture systems. This protocol for optimization of tissue cyst purification from the brains of infected mice using Percoll gradients provides an efficient means to recover in vivo\u2013derived tissue cysts that can be applied to imaging, cell biological, biochemical, transcriptomic, and proteomic analyses. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.30","type":"journal-article","created":{"date-parts":[[2017,5,16]],"date-time":"2017-05-16T14:07:19Z","timestamp":1494943639000},"source":"Crossref","is-referenced-by-count":21,"title":["Purification <i>Toxoplasma gondii<\/i> Tissue Cysts Using Percoll Gradients"],"prefix":"10.1002","volume":"45","author":[{"given":"Elizabeth A.","family":"Watts","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine  Lexington Kentucky"},{"name":"Current Address: Department of Biochemistry and Molecular Biology, University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Animesh","family":"Dhara","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine  Lexington Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony P.","family":"Sinai","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine  Lexington Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,5,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0032489"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2011.10.004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000050071"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005643"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02091.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284258"},{"key":"e_1_2_7_8_1","first-page":"1019","article-title":"Advances in the life cycle of Toxoplasma gondii","volume":"28","author":"Dubey J. 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