{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T06:20:53Z","timestamp":1788589253687,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T00:00:00Z","timestamp":1502755200000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2017,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The apicomplexan parasite <jats:italic>Cryptosporidium<\/jats:italic> is a leading cause of diarrheal disease and an important contributor to overall global child mortality. We currently lack effective treatment and immune prophylaxis. Recent advances now permit genetic modification of this important pathogen. We expect this to produce rapid advances in fundamental as well as translational research on cryptosporidiosis. Here we outline genetic engineering for <jats:italic>Cryptosporidium<\/jats:italic> in sufficient detail to establish transfection in any laboratory that requires access to this key technology. This chapter details the conceptual design consideration, as well as the experimental steps required to transfect, select, and isolate transgenic parasites. We also provide detail on key in vitro and in vivo assays to detect, validate, and quantify genetically modified <jats:italic>Cryptosporidium<\/jats:italic> parasites. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.33","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T13:53:16Z","timestamp":1502459596000},"source":"Crossref","is-referenced-by-count":72,"title":["Generating and Maintaining Transgenic <i>Cryptosporidium parvum<\/i> Parasites"],"prefix":"10.1002","volume":"46","author":[{"given":"Mattie C.","family":"Pawlowic","sequence":"first","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumiti","family":"Vinayak","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Sateriale","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carrie F.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Striepen","sequence":"additional","affiliation":[{"name":"Center for Tropical and Emerging Global Diseases, University of Georgia  Athens Georgia"},{"name":"Department of Cellular Biology, University of Georgia  Athens Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,15]]},"reference":[{"key":"e_1_2_16_2_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1094786"},{"key":"e_1_2_16_2_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)11401-2"},{"key":"e_1_2_16_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.12.002"},{"key":"e_1_2_16_2_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00387-13"},{"key":"e_1_2_16_2_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70772-8"},{"key":"e_1_2_16_2_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91478-S"},{"key":"e_1_2_16_2_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00731-16"},{"key":"e_1_2_16_2_8_1","doi-asserted-by":"publisher","DOI":"10.2307\/3283119"},{"key":"e_1_2_16_2_9_1","volume-title":"The general biology of Cryptosporidium","author":"Fayer R","year":"1997"},{"key":"e_1_2_16_2_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00357-08"},{"issue":"9","key":"e_1_2_16_2_11_1","doi-asserted-by":"crossref","first-page":"2503","DOI":"10.1128\/JCM.36.9.2503-2508.1998","article-title":"The gamma interferon gene knockout mouse: A highly sensitive model for evaluation of therapeutic agents against Cryptosporidium parvum","volume":"36","author":"Griffiths J. 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