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An obligate intracellular pathogen, <jats:italic>Coxiella burnetii<\/jats:italic> survives within a large, lysosome\u2010like vacuole inside the host cell. A unique feature of the <jats:italic>Coxiella<\/jats:italic> parasitophorous vacuole (PV) is high levels of fusion with the host endocytic pathway, with PV\u2010endosome fusion critical for <jats:italic>Coxiella<\/jats:italic> survival within the host cell. This unit describes quantitating PV\u2010endosome fusion by measuring delivery of the fluid phase endosome marker dextran to the PV using live cell imaging. To study the effect of host cell proteins involved in PV\u2010endosome fusion, details are provided for using siRNA knockdown host cells. This method is a powerful tool for understanding mechanisms underlying <jats:italic>Coxiella<\/jats:italic>\u2019s ability to manipulate host cell trafficking pathways. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.34","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T13:53:16Z","timestamp":1502459596000},"source":"Crossref","is-referenced-by-count":4,"title":["Quantitative Dextran Trafficking to the <i>Coxiella burnetii<\/i> Parasitophorous Vacuole"],"prefix":"10.1002","volume":"46","author":[{"given":"Seth","family":"Winfree","sequence":"first","affiliation":[{"name":"Department of Medicine Division of Nephrology Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stacey D.","family":"Gilk","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,8,15]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5816-5821.2002"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12087"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00688-10"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.12.015"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005915"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.3.796-809.1996"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12637"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.7.1792-1799.1988"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1309195110"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb-2016-0044"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201004104"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00606-12"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc11a02s27"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02313-16"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-007-4315-1_11"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2006.00838.x"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc06c01s15"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12494"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.2469-2477.2005"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.34","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.34","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.34","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.34","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T12:51:20Z","timestamp":1693745480000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.34"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,8]]}},"alternative-id":["10.1002\/cpmc.34"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.34","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8]]}}}