{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:09:04Z","timestamp":1790834944308,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T00:00:00Z","timestamp":1510185600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>\n<jats:italic>Coxiella burnetii<\/jats:italic> is the causative agent of human Q fever, a zoonotic disease that can cause a debilitating, flu\u2010like illness in acute cases, or a life\u2010threatening endocarditis in chronic patients. An obligate intracellular bacterial pathogen, <jats:italic>Coxiella<\/jats:italic> survives and multiplies in a large lysosome\u2010like vacuole known as the <jats:italic>Coxiella<\/jats:italic> parasitophorous vacuole (CPV). A unique characteristic of the CPV is the acidic environment (pH \u223c5.0), which is required to activate <jats:italic>Coxiella<\/jats:italic> metabolism and the <jats:italic>Coxiella<\/jats:italic> type 4 secretion system (T4SS), a major virulence factor required for intracellular survival. Further, inhibiting or depleting vacuolar ATPase, a host cell protein that regulates lysosomal pH, inhibits intracellular <jats:italic>Coxiella<\/jats:italic> growth. Together, these data suggest that CPV pH is an important limiting factor for <jats:italic>Coxiella<\/jats:italic> growth and virulence. This unit describes a method to determine CPV pH using live cell microscopy of a pH\u2013sensitive fluorophore conjugated to dextran. This technique is useful to measure changes in CPV pH during infection or in response to drug treatment. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.38","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T16:46:21Z","timestamp":1510245981000},"source":"Crossref","is-referenced-by-count":9,"title":["Measuring pH of the <i>Coxiella burnetii<\/i> Parasitophorous Vacuole"],"prefix":"10.1002","volume":"47","author":[{"given":"Dhritiman","family":"Samanta","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacey D.","family":"Gilk","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Indiana University School of Medicine  Indianapolis Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/PL00006177"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5816-5821.2002"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.mcb.2014.10.021"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac8011347"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2005.00360.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.8.4488"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1462-5822.2002.00191.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2005.00527.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.5.3240"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.64.3.796-809.1996"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00406-10"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201507112"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00112"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.12.5013-5016.1992"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00606-12"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02313-16"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054566"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01911-07"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0812074106"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo9706178"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2007.00901.x"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.34"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.38","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.38","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.38","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.38","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T06:28:01Z","timestamp":1693722481000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.38"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/cpmc.38"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.38","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]}}}