{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T05:41:08Z","timestamp":1693806068381},"reference-count":17,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T00:00:00Z","timestamp":1510185600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Molluscum contagiosum virus (MCV) is a common skin pathogen of children and young adults. Infection with MCV causes benign skin tumors in children and young adults and is mostly self\u2010limiting. In contrast to orthopoxviruses, MCV infections tend to take a subacute clinical course but may persist for up to 12 months. Current numbers for MCV seroprevalence in different geographical areas are based on a variety of historical serological methods from complement fixation assays to MCV ELISAs based on purified MCV virions and MC133 antigen expressed in a Semliki Forest Virus expression system. A standardized ELISA for the assessment of MCV seroprevalence would be useful to determine global MCV seroprevalence. The methods described show that polypeptides derived from MCV open reading frames MC084 (residues V123 to R230 and V33 to G117), mc133 (residues M1 to N370), and glutathione S\u2010transferase (GST)\u2010H3L (residues I142 to W251) expressed in <jats:italic>E.\u00a0coli<\/jats:italic> RIL+ as GST fusion proteins can be used to assess antibody binding in a GST capture ELISA. We show how the ELISA can be used to screen a panel of patient sera previously characterized with the mc084 V123\u2010R230 ELISA. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.42","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T16:46:21Z","timestamp":1510245981000},"source":"Crossref","is-referenced-by-count":1,"title":["ELISA for Molluscum Contagiosum Virus"],"prefix":"10.1002","volume":"47","author":[{"given":"Subuhi","family":"Sherwani","sequence":"first","affiliation":[{"name":"Department of Microbiology, Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cardiff University School of Medicine  Cardiff United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim J.","family":"Bugert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cardiff University School of Medicine  Cardiff United Kingdom"},{"name":"Institut f\u00fcr Mikrobiologie der Bundeswehr  M\u00fcnchen Deutschland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70109-9"},{"key":"e_1_2_7_3_1","unstructured":"Chowdhury M.(2015).Construction of pGEX6P1\u2010GST\u2010H3L (I142\u2010W251) fusion vector plasmid and evaluation as a GST fusion protein in the MCV ELISA(Unpublished thesis). Cardiff University Cardiff United Kingdom."},{"key":"e_1_2_7_4_1","first-page":"293","article-title":"Tasks in the wake of smallpox eradication","volume":"8","author":"Jezek Z.","year":"1987","journal-title":"World Health Forum"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/314620"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166-0934(92)90067-N"},{"key":"e_1_2_7_7_1","first-page":"A.3A.1","article-title":"Assays for total protein","volume":"00","author":"Lovrien R.","year":"2005","journal-title":"Current Protocols in Microbiology"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mca03ms22"},{"key":"e_1_2_7_9_1","first-page":"371","article-title":"Preparation and use of molluscum contagiosum virus from human tissue biopsy specimens","volume":"269","author":"Melquiot N. V.","year":"2004","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_7_10_1","first-page":"44","article-title":"Observations on the virus of molluscum contagiosum","volume":"34","author":"Mitchell J. C.","year":"1952","journal-title":"British Journal of Experimental Pathology"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5276.813"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-876-4_8"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088734"},{"key":"e_1_2_7_14_1","unstructured":"Sherwani S.(2013).Investigation of the infectious cycle of molluscum contagiosum virus in human skin and the nature of MCV induced immunity(Unpublished doctoral dissertation). Cardiff University Cardiff United Kingdom. doi:Orca.cf.ac.uk\/57429\/."},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1979.tb05597.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mca04as00"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1086\/514197"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.136.12.1518"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.42","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.42","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.42","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.42","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T06:27:55Z","timestamp":1693722475000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.42"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":17,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/cpmc.42"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.42","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]}}}