{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T11:47:48Z","timestamp":1694951268923},"reference-count":6,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T00:00:00Z","timestamp":1510185600000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This protocol describes a method for the rapid detection of leptospiral DNA in environmental water. In summary, the DNA is extracted from water samples and tested in a TaqMan\u2010based real\u2010time quantitative polymerase chain reaction (qPCR) for the presence of <jats:italic>lipl32<\/jats:italic>, a gene that is present only in pathogenic <jats:italic>Leptospira<\/jats:italic> spp. The gene target used in this assay is important in that it only detects pathogenic leptospires and not the saprophytic leptospires that may be present in environmental samples. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.43","type":"journal-article","created":{"date-parts":[[2017,11,9]],"date-time":"2017-11-09T16:46:21Z","timestamp":1510245981000},"source":"Crossref","is-referenced-by-count":2,"title":["<i>Leptospira<\/i>: Molecular Detection of Pathogenic Species in Natural Sources"],"prefix":"10.1002","volume":"47","author":[{"given":"Brittney","family":"Beigel","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Lincoln Memorial University  Harrogate Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashutosh","family":"Verma","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Lincoln Memorial University  Harrogate Tennessee"},{"name":"Center for Infectious, Zoonotic and Vector\u2010borne Diseases, College of Veterinary Medicine, Lincoln Memorial University  Harrogate Tennessee"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,9]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2208"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph110807953"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2009.03.014"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45860-0"},{"key":"e_1_2_7_7_1","first-page":"12E.1.1","article-title":"Laboratory maintenance of pathogenic Leptospira","volume":"00","author":"Zuerner R. L.","year":"2005","journal-title":"Current Protocols in Microbiology"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.43","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.43","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.43","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.43","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T06:28:00Z","timestamp":1693722480000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.43"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11]]},"references-count":6,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1002\/cpmc.43"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.43","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11]]}}}