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Furthermore, NDV has modular genome and accommodates insertion of a foreign gene as a transcriptional unit, thus enabling NDV as a vaccine vector against diseases of humans and animals. Avirulent NDV strains (e.g., LaSota and B1) have been commonly used as vaccine vectors. In this protocol, we have described reverse genetics of NDV to be used as a vaccine vector by exemplifying the recovery of NDV vectored avian influenza virus vaccine. Specifically, cloning and recovery of NDV expressing the hemagglutinin protein of highly pathogenic influenza virus were explained. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.44","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T05:51:11Z","timestamp":1519365071000},"source":"Crossref","is-referenced-by-count":14,"title":["Reverse Genetics for Newcastle Disease Virus as a Vaccine Vector"],"prefix":"10.1002","volume":"48","author":[{"given":"Shin\u2010Hee","family":"Kim","sequence":"first","affiliation":[{"name":"VA\u2010MD College of Veterinary Medicine, University of Maryland  College Park Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siba K.","family":"Samal","sequence":"additional","affiliation":[{"name":"VA\u2010MD College of Veterinary Medicine, University of Maryland  College Park Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,22]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Alexander D. 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