{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T23:08:55Z","timestamp":1768259335317,"version":"3.49.0"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T00:00:00Z","timestamp":1519257600000},"content-version":"vor","delay-in-days":52,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Poliovirus is a prototype member of the <jats:italic>Enterovirus<\/jats:italic> genus of the <jats:italic>Picornaviridae<\/jats:italic> family of small positive strand RNA viruses, which include important human and animal pathogens. Quantitative assessment of viral replication is very important for investigation of the virus biology and the development of anti\u2010viral strategies. The poliovirus genome structure allows replacement of structural genes with a reporter protein, such as a luciferase or a fluorescent protein, whose signals can be detected and quantified <jats:italic>in vivo<\/jats:italic>, thus permitting observation of replication kinetics in live cells. This paper presents protocols for poliovirus replicon RNA production, purification, packaging and transfection, as well as techniques for monitoring <jats:italic>Renilla<\/jats:italic> luciferase replication signal in living cells. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.47","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T05:51:11Z","timestamp":1519365071000},"source":"Crossref","is-referenced-by-count":16,"title":["Poliovirus Replicon RNA Generation, Transfection, Packaging, and Quantitation of Replication"],"prefix":"10.1002","volume":"48","author":[{"given":"Ekaterina G.","family":"Viktorova","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, Virginia\u2010Maryland Regional College of Veterinary Medicine, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sunil","family":"Khattar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Virginia\u2010Maryland Regional College of Veterinary Medicine, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siba","family":"Samal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Virginia\u2010Maryland Regional College of Veterinary Medicine, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George A.","family":"Belov","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Virginia\u2010Maryland Regional College of Veterinary Medicine, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.08.007"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01820-06"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.11.7207-7216.2005"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc15h01s29"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/369648"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00594"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.14.6394-6400.2000"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/104303401753153893"},{"issue":"5","key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"1687","DOI":"10.1128\/jvi.62.5.1687-1696.1988","article-title":"Construction and Characterization of Poliovirus Subgenomic Replicons","volume":"62","author":"Kaplan G.","year":"1988","journal-title":"Journal of Virology"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc15f02s01"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.1.427-435.1999"},{"issue":"8","key":"e_1_2_8_13_1","doi-asserted-by":"crossref","first-page":"5040","DOI":"10.1128\/jvi.66.8.5040-5046.1992","article-title":"A Poliovirus Replicon Containing the Chloramphenicol Acetyltransferase Gene Can Be Used to Study the Replication and Encapsidation of Poliovirus Rna","volume":"66","author":"Percy N.","year":"1992","journal-title":"Journal of Virology"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1998.9046"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpmc.47","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.47","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.47","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.47","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T14:50:44Z","timestamp":1693752644000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.47"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1]]},"references-count":13,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,1]]}},"alternative-id":["10.1002\/cpmc.47"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.47","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1]]}}}