{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T10:43:17Z","timestamp":1778236997017,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,2,22]],"date-time":"2018-02-22T00:00:00Z","timestamp":1519257600000},"content-version":"vor","delay-in-days":52,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hepatitis E virus (HEV) predominantly causes acute liver disease in humans and is transmitted via the fecal\u2010oral route. HEV infection in pregnant women can result in grave consequences, with up to 30% fatality. The HEV strains infecting humans mainly belong to four genotypes. Genotypes 1 and 2 are restricted to human infection, while genotypes 3 and 4 are zoonotic. HEV genotype 3 (HEV\u20103) can cause both acute and chronic liver diseases. Several cell lines (mainly hepatocytes) have been developed for HEV propagation and biological study. However, HEV production in these cell lines is suboptimal and inefficient. Here, we present methods for the isolation, propagation, and quantification of HEV. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.50","type":"journal-article","created":{"date-parts":[[2018,2,23]],"date-time":"2018-02-23T05:51:11Z","timestamp":1519365071000},"source":"Crossref","is-referenced-by-count":2,"title":["Hepatitis E Virus: Isolation, Propagation, and Quantification"],"prefix":"10.1002","volume":"48","author":[{"given":"Shaoli","family":"Lin","sequence":"first","affiliation":[{"name":"Molecular Virology Laboratory, VA\u2010MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Yang","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, VA\u2010MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Jin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, VA\u2010MD College of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland  College Park Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13062-015-0050-0"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc01a01s13"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1614955114"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.9.4838-4846.2004"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00593-10"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.251555098"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00046-06"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1204512"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90230-M"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)61849-7"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2015.10.048"},{"key":"e_1_2_8_13_1","article-title":"Hepatitis E virus lifecycle and identification of 3 forms of the ORF2 capsid protein","author":"Montpellier C.","year":"2017","journal-title":"Gastroenterology"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2015.06.006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01419"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.02.002"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.678"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-012-0682-0"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-9-28"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2007.12.008"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.27990"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00146-12"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1018878108"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.068429-0"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18040869"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82535-0"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1290\/1071-2690(2002)038<0079:CPAFDO>2.0.CO;2"},{"key":"e_1_2_8_28_1","first-page":"2.5A.1","article-title":"Agarose gel electrophoresis","volume":"51","author":"Voytas D.","year":"2001","journal-title":"Current Protocols in Molecular Biology"},{"key":"e_1_2_8_29_1","unstructured":"World Health Organization. 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