{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:38:56Z","timestamp":1786977536765,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T00:00:00Z","timestamp":1526601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Coxiella burnetii<\/jats:italic>\n                    is a highly infectious obligate intracellular bacterium and the etiological agent of the zoonosis Query (Q) fever. This Gram\u2010negative gamma\u2010proteobacterium has adapted to replicate within a specialized compartment in mammalian phagocytic cells, known as the\n                    <jats:italic>Coxiella<\/jats:italic>\n                    \u2010containing vacuole (CCV). Knowledge of critical characteristics of the CCV microenvironment (e.g., luminal pH), analysis of the\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    genome sequence, and strategic metabolic profiling have provided the basis for determining the physicochemical and nutritional conditions necessary to support axenic replication of\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    . In this unit, the media currently utilized for axenic culture of\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    are described, with emphasis on application. To aid in experimental reproducibility and interpretation of results, considerations and limitations are discussed. Lastly, expected results for\n                    <jats:italic>C. burnetii<\/jats:italic>\n                    axenic growth under control conditions are provided as a reference. \u00a9 2018 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cpmc.52","type":"journal-article","created":{"date-parts":[[2018,5,18]],"date-time":"2018-05-18T11:53:53Z","timestamp":1526644433000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Use of Axenic Culture Tools to Study\n                    <i>Coxiella burnetii<\/i>"],"prefix":"10.1002","volume":"50","author":[{"given":"Savannah E.","family":"Sanchez","sequence":"first","affiliation":[{"name":"Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University  Pullman Washington"},{"name":"School of Molecular Biosciences, College of Veterinary Medicine, Washington State University  Pullman Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Vallejo\u2010Esquerra","sequence":"additional","affiliation":[{"name":"Paul G. Allen School for Global Animal Health, College of Veterinary Medicine, Washington State University  Pullman Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anders","family":"Omsland","sequence":"additional","affiliation":[{"name":"Paul G. 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