{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T12:23:18Z","timestamp":1787314998963,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:00:00Z","timestamp":1524787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Mucor circinelloides<\/jats:italic>\n                    is a fungus that belongs to the order Mucorales. It grows as mold in the environment and can cause mucormycosis, a potentially fatal infection in immunocompromised patients.\n                    <jats:italic>M. circinelloides<\/jats:italic>\n                    is a biodiesel producer and serves as a model organism for studying several biological processes, such as light responses and RNA interference\u2013mediated gene silencing. Over the past decade, the increasing number of molecular tools has also allowed us to manipulate the genome of this fungus. This article outlines the fundamental protocols for the\n                    <jats:italic>in vitro<\/jats:italic>\n                    growth, maintenance, and genetic manipulation of\n                    <jats:italic>M. circinelloides<\/jats:italic>\n                    in the laboratory. \u00a9 2018 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cpmc.53","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T16:36:53Z","timestamp":1524847013000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["<i>Mucor circinelloides<\/i>\n                    : Growth, Maintenance, and Genetic Manipulation"],"prefix":"10.1002","volume":"49","author":[{"given":"Sandeep","family":"Vellanki","sequence":"first","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases (STCEID), Department of Biology, The University of Texas at San Antonio  Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Isabel","family":"Navarro\u2010Mendoza","sequence":"additional","affiliation":[{"name":"Departmento de Genetica y Microbiologia, Facultad de Biologia, Universidad de Murcia  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexis","family":"Garcia","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases (STCEID), Department of Biology, The University of Texas at San Antonio  Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Murcia","sequence":"additional","affiliation":[{"name":"Departmento de Genetica y Microbiologia, Facultad de Biologia, Universidad de Murcia  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Perez\u2010Arques","sequence":"additional","affiliation":[{"name":"Departmento de Genetica y Microbiologia, Facultad de Biologia, Universidad de Murcia  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoriano","family":"Garre","sequence":"additional","affiliation":[{"name":"Departmento de Genetica y Microbiologia, Facultad de Biologia, Universidad de Murcia  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco E.","family":"Nicolas","sequence":"additional","affiliation":[{"name":"Departmento de Genetica y Microbiologia, Facultad de Biologia, Universidad de Murcia  Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Chan","family":"Lee","sequence":"additional","affiliation":[{"name":"South Texas Center for Emerging Infectious Diseases (STCEID), Department of Biology, The University of Texas at San Antonio  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