{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:44:53Z","timestamp":1753868693707,"version":"3.41.2"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T00:00:00Z","timestamp":1524787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Infection by DNA viruses such as human adenoviruses (HAdVs) causes a high\u2010level accumulation of viral DNA and mRNA in the cell population. However, the average viral DNA and mRNA content in a heterogeneous cell population does not inevitably reflect the abundance in individual cells. As the vast majority of virus infection studies is carried out using standard experimental procedures with heterogeneous cell populations, there is a need for a method allowing simultaneous detection and quantitative analysis of viral genome accumulation and gene expression in individual infected cells within a population. This article describes a padlock probe\u2013based rolling\u2010circle amplification protocol that allows simultaneous detection of HAdV type 5 (HAdV\u20105) DNA and various virus\u2010encoded mRNAs, as well as quantitative analysis of HAdV\u20105 DNA copies and mRNA species, in individual cells within a heterogeneous population. This versatile method can be used to detect the extent of pathogenic DNA virus infection in different cell types over prolonged infection times. Furthermore, simultaneous viral DNA and mRNA quantification in individual cells allows identification of cells in which persistent infections may be established. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.54","type":"journal-article","created":{"date-parts":[[2018,4,27]],"date-time":"2018-04-27T20:36:52Z","timestamp":1524861412000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In Situ Detection of Adenovirus DNA and mRNA in Individual Cells"],"prefix":"10.1002","volume":"49","author":[{"given":"Tanel","family":"Punga","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Microbiology, Uppsala University  Uppsala Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sibel","family":"Ciftci","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mats","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomasz","family":"Krzywkowski","sequence":"additional","affiliation":[{"name":"Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University  Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,4,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2741\/3059"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0154814"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.3820805"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/S0168-1702(95)00071-2","article-title":"DNA sequence of the deletion\/insertion in early region 3 of Ad5 dl309","volume":"39","author":"Bett A. 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