{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T13:54:23Z","timestamp":1778680463198,"version":"3.51.4"},"reference-count":16,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T00:00:00Z","timestamp":1527120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Shigella<\/jats:italic> is an enteroinvasive human pathogen that infects the colonic epithelium and causes Shigellosis, an infectious diarrheal disease. There is no vaccine for the prevention or treatment of Shigellosis and antibiotic\u2010resistant strains of <jats:italic>Shigella<\/jats:italic> are increasing, emphasizing the need for a deeper understanding of <jats:italic>Shigella<\/jats:italic> pathogenesis in order to design effective antimicrobial therapies. Small animal models do not recapitulate Shigellosis, therefore tissue\u2010cultured cells have served as model systems to study <jats:italic>Shigella<\/jats:italic> pathogenesis. Here, protocols to enumerate <jats:italic>Shigella<\/jats:italic> invasion, cell\u2010cell spread, and plaque formation in the tissue\u2010cultured cell lines Henle\u2010407 and CoN\u2010841 are described. Additionally, a new method to study <jats:italic>Shigella<\/jats:italic> invasion in primary intestinal enteroids is described. These protocols can be used to examine different aspects of <jats:italic>Shigella<\/jats:italic> virulence. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.57","type":"journal-article","created":{"date-parts":[[2018,5,24]],"date-time":"2018-05-24T19:03:52Z","timestamp":1527188632000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>Shigella<\/i> Pathogenesis Modeling with Tissue Culture Assays"],"prefix":"10.1002","volume":"50","author":[{"given":"Benjamin J.","family":"Koestler","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin  Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cara M.","family":"Ward","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin  Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shelley M.","family":"Payne","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin  Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,5,24]]},"reference":[{"key":"e_1_2_11_2_1_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3867"},{"key":"e_1_2_11_2_2_1","unstructured":"FDA. 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