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The medical impact of\n                    <jats:italic>C. albicans<\/jats:italic>\n                    depends, in part, on its ability to form biofilms, communities of adhered cells encased in an extracellular matrix. Biofilms can form on both biotic and abiotic surfaces, such as tissues and implanted medical devices. Once formed, biofilms are highly resistant to antifungal agents and the host immune system, and can act as a protected reservoir to seed disseminated infections. Here, we present several\n                    <jats:italic>in vitro<\/jats:italic>\n                    biofilm protocols, including protocols that are optimized for high\u2010throughput screening of mutant libraries and antifungal compounds. We also present protocols to examine specific stages of biofilm development and protocols to evaluate interspecies biofilms that\n                    <jats:italic>C. albicans<\/jats:italic>\n                    forms with interacting microbial partners. \u00a9 2018 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cpmc.60","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T11:10:48Z","timestamp":1531307448000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":131,"title":["<i>In Vitro<\/i>\n                    Culturing and Screening of\n                    <i>Candida albicans<\/i>\n                    Biofilms"],"prefix":"10.1002","volume":"50","author":[{"given":"Megha","family":"Gulati","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew B.","family":"Lohse","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of California, San Francisco  San Francisco California"},{"name":"Department of Biology, BioSynesis, Inc.  San Francisco California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig L.","family":"Ennis","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"},{"name":"Quantitative and Systems Biology Graduate Program, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth E.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Austin M.","family":"Perry","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"},{"name":"Quantitative and Systems Biology Graduate Program, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Bapat","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"},{"name":"Quantitative and Systems Biology Graduate Program, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashley Valle","family":"Arevalo","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"},{"name":"Quantitative and Systems Biology Graduate Program, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana L.","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"},{"name":"Quantitative and Systems Biology Graduate Program, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clarissa J.","family":"Nobile","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, School of Natural Sciences, University of California, Merced  Merced California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,11]]},"reference":[{"key":"e_1_2_23_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.10.6023-6031.2004"},{"key":"e_1_2_23_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-48-7-671"},{"issue":"1","key":"e_1_2_23_4_1","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1128\/iai.26.1.348-354.1979","article-title":"Effects of low concentrations of zinc on the growth and dimorphism of Candida albicans: Evidence for zinc\u2010resistant and \u2010sensitive pathways for mycelium formation","volume":"26","author":"Bedell G. 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