{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:44:54Z","timestamp":1753868694553,"version":"3.41.2"},"reference-count":13,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,10,3]],"date-time":"2018-10-03T00:00:00Z","timestamp":1538524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Ectromelia virus (ECTV) is an orthopoxvirus that causes mousepox in mice. Members of the genus orthopoxvirus are closely related and include variola (the causative agent of smallpox in humans), monkeypox, and vaccinia. Common features of variola virus and ECTV further include a restricted host range and similar disease progression in their respective hosts. Mousepox makes an excellent small animal model for smallpox to investigate pathogenesis, vaccine and antiviral agent testing, host\u2010virus interactions, and immune and inflammatory responses. The availability of a wide variety of inbred, congenic, and gene\u2010knockout mice allows detailed analyses of the host response. ECTV mutant viruses lacking one or more genes encoding immunomodulatory proteins are being used in numerous studies in conjunction with wild\u2010type or gene\u2010knockout mice to study the functions of these genes in host\u2010virus interactions. The methods used for propagation of ECTV in cell culture, purification, and quantification of infectious particles through viral plaque assay are described. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.65","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T15:01:10Z","timestamp":1538751670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Propagation and Purification of Ectromelia Virus"],"prefix":"10.1002","volume":"51","author":[{"given":"Geeta","family":"Chaudhri","sequence":"first","affiliation":[{"name":"Research School of Population Health, College of Health and Medicine, Australian National University  Canberra Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Georgina","family":"Kaladimou","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Health and Medicine, University of Tasmania  Hobart Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pratikshya","family":"Pandey","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Health and Medicine, University of Tasmania  Hobart Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunasegaran","family":"Karupiah","sequence":"additional","affiliation":[{"name":"School of Medicine, College of Health and Medicine, University of Tasmania  Hobart Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80030-1"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005175"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(03)00520-8"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc14a03s39"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81090-0"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(48)91599-2"},{"issue":"4","key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.4049\/jimmunol.63.4.341","article-title":"Mouse\u2010pox; infectious ectromelia of mice; a review","volume":"63","author":"Fenner F.","year":"1949","journal-title":"Journal of Immunology"},{"volume-title":"Smallpox and its eradication","year":"1988","author":"Fenner F.","key":"e_1_2_8_9_1"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(62)90172-1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1700330317"},{"issue":"3","key":"e_1_2_8_12_1","first-page":"325","article-title":"Studies on filterable viruses: I. Cultivation of vaccine virus","volume":"1","author":"Parker F.","year":"1925","journal-title":"The American Journal of Pathology"},{"key":"e_1_2_8_13_1","first-page":"163","article-title":"Techniques for studying murine natural killer cells in defense against viral infection","volume":"121","author":"Scalzo A. 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