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The public health relevance of these pathogens has galvanized extensive research over the past several decades and led to characterization of their sexual cycles. This research has allowed several <jats:italic>Cryptococcus<\/jats:italic> species to develop into model fungal organisms for both pathogenesis and basic science studies. Many of these studies require observation of the meiotic process and its associated mating structures as well as generation of meiotic progeny with novel phenotypes and genotypes. Herein, we describe how to set up genetic crosses between <jats:italic>Cryptococcus<\/jats:italic> strains and observe their mating phenotypes as well as how to recover progeny from these crosses for further analysis. \u00a9 2019 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.75","type":"journal-article","created":{"date-parts":[[2019,1,20]],"date-time":"2019-01-20T12:49:31Z","timestamp":1547988571000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["<i>Cryptococcus neoformans<\/i> Mating and Genetic Crosses"],"prefix":"10.1002","volume":"53","author":[{"given":"Sheng","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Molecular Genetics and Microbiology Duke University Medical Center  Durham North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelby J.","family":"Priest","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology Duke University Medical Center  Durham North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Heitman","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology Duke University Medical Center  Durham North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,1,20]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00352-08"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000850"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1999.1180"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.06.003"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.117.300656"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00868-15"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1003688"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002528"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.2.5.1036-1045.2003"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2015.02.009"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/fgbi.1999.1155"},{"key":"e_1_2_11_13_1","volume-title":"Cryptococcus : from human pathogen to model yeast.","author":"Heitman J.","year":"2011"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/015594-0"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.68"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1041402"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.109.113027"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1245"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004261"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.59"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7757"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00970-08"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1080\/00275514.1975.12019866"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.2307\/3758800"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.2307\/3758813"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00357-16"},{"key":"e_1_2_11_27_1","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1128\/iai.60.2.602-605.1992","article-title":"Genetic association of mating types and virulence in Cryptococcus neoformans","volume":"60","author":"Kwon\u2010Chung K. 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