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In 2016, there were 280,000 cases of cryptococcal meningitis in the HIV+ population, two\u2010thirds of them fatal; other immunocompromised patients are also affected. The burden of cryptococcal disease and the limits of current chemotherapy create a pressing need for improved treatment. One hindrance to the development of new therapies is lack of understanding of how this pathogen breaches the barriers protecting the brain. Here we describe a tool for investigating this process. This simple in vitro blood\u2010brain\u2010barrier (BBB) model, based on a human brain endothelial cell line grown on a permeable membrane, may be used to assay the BBB transmigration of <jats:italic>C. neoformans<\/jats:italic> or other neurotropic pathogens. \u00a9 2019 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmc.78","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T19:49:35Z","timestamp":1550519375000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["An In Vitro Brain Endothelial Model for Studies of Cryptococcal Transmigration into the Central Nervous System"],"prefix":"10.1002","volume":"53","author":[{"given":"Felipe H.","family":"Santiago\u2010Tirado","sequence":"first","affiliation":[{"name":"Department of Molecular Microbiology Washington University School of Medicine  St. Louis Missouri"},{"name":"Current address: Department of Biological Sciences University of Notre Dame  Notre Dame Indiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robyn S.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Medicine Washington University School of Medicine  St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara L.","family":"Doering","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology Washington University School of Medicine  St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof3040057"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mc01a01s13"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.9.4985-4995.2004"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.05230-0"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a020412"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020758"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.6.3642-3650.2000"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2007.146852"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0271678X16630991"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3656"},{"key":"e_1_2_9_12_1","doi-asserted-by":"crossref","first-page":"602","DOI":"10.1128\/iai.60.2.602-605.1992","article-title":"Genetic association of mating types and virulence in Cryptococcus neoformans","volume":"60","author":"Kwon\u2010Chung K. 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