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It is ubiquitous in the environment and is first inhaled into the lungs of the host, where it is taken up by phagocytes. The interaction of these fungal cells with host phagocytes, therefore, is a critical step in the pathogenesis of this disease. One characteristic of this initial step in host\u2013pathogen interactions is the avidity with which fungal cells are taken up by phagocytes, described by the phagocytic index. In this chapter, we detail a high\u2010throughput method of directly assessing the phagocytic index of fungal cells using an imaging\u2010based paradigm. By automating image collection and processing, this method permits rapid assessment of this critical host interaction. \u00a9 2019 by John Wiley &amp; Sons, Inc.\n                  <\/jats:p>","DOI":"10.1002\/cpmc.79","type":"journal-article","created":{"date-parts":[[2019,2,26]],"date-time":"2019-02-26T00:36:57Z","timestamp":1551141417000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["An Automated Assay to Measure Phagocytosis of\n                    <i>Cryptococcus neoformans<\/i>"],"prefix":"10.1002","volume":"53","author":[{"given":"Andrew L.","family":"Chang","sequence":"first","affiliation":[{"name":"Washington University in St. Louis  St. Louis Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camaron R.","family":"Hole","sequence":"additional","affiliation":[{"name":"Washington University in St. Louis  St. Louis 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