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The fungus was long considered a purely asexual organism. However, a sexual cycle was reported in 2009, with methods described to induce mating under laboratory conditions. The presence of a sexual cycle now offers a valuable tool for classical genetic analysis of the fungus, such as allowing determination of whether traits of interest are mono\u2010 or poly\u2010genic in nature. For example, the sexual cycle is currently being exploited to determine the genetic basis of traits of medical importance such as resistance to azole antifungals and virulence, and to characterize the genes involved. The sexual cycle can also be used to assess the possibility of gene flow between isolates. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.<\/jats:p><jats:p>This unit describes protocols for culturing of <jats:italic>A. fumigatus<\/jats:italic> and for inducing sexual reproduction between compatible <jats:italic>MAT1\u20101<\/jats:italic> and <jats:italic>MAT1\u20102<\/jats:italic> isolates of the species. The unit also provides working methods for harvesting sexual structures, isolating single\u2010spore progeny and confirming whether sexual recombination has occurred. \u00a9 The Authors. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.<\/jats:p>","DOI":"10.1002\/cpmc.87","type":"journal-article","created":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T15:37:27Z","timestamp":1567352247000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Culturing and Mating of <i>Aspergillus fumigatus<\/i>"],"prefix":"10.1002","volume":"54","author":[{"given":"George D.","family":"Ashton","sequence":"first","affiliation":[{"name":"School of Life Sciences University of Nottingham  Nottingham United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul S.","family":"Dyer","sequence":"additional","affiliation":[{"name":"School of Life Sciences University of Nottingham  Nottingham United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,7]]},"reference":[{"key":"e_1_2_11_2_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2018.08.004"},{"key":"e_1_2_11_2_2_1","first-page":"66","volume-title":"Experiments in microbiology, plant pathology and biotechnology","author":"Aneja K. 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