{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T01:12:11Z","timestamp":1780449131792,"version":"3.54.1"},"reference-count":8,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T00:00:00Z","timestamp":1566259200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Microbiology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Aspergillus fumigatus<\/jats:italic> is an opportunistic human pathogenic mold. DNA extraction from this fungus is usually performed by mechanical perturbation of cells, as it possesses a rigid and complex cell wall. While this is not problematic for single isolates, it can be time consuming for large numbers of strains if using traditional DNA extraction procedures. Therefore, in this article we describe a fast and efficient thermal\u2010shock method to release DNA from spores of <jats:italic>A. fumigatus<\/jats:italic> and other filamentous fungi without the need for complex extraction methods. This is especially important for high\u2010throughput PCR analyses of mutants in 96\u2010 or 384\u2010well formats in a very short period of time without any concern about sample cross\u2010contamination. This method is currently being used to validate the protein\u2010coding gene and non\u2010coding RNA knockout libraries in <jats:italic>A. fumigatus<\/jats:italic> generated in our laboratory, and could be used in the future for diagnostics purposes. \u00a9 2019 The Authors.<\/jats:p>","DOI":"10.1002\/cpmc.89","type":"journal-article","created":{"date-parts":[[2019,8,20]],"date-time":"2019-08-20T13:16:52Z","timestamp":1566307012000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Fast and Reliable PCR Amplification from <i>Aspergillus fumigatus<\/i> Spore Suspension Without Traditional DNA Extraction"],"prefix":"10.1002","volume":"54","author":[{"given":"Marcin G.","family":"Fraczek","sequence":"first","affiliation":[{"name":"Manchester Institute of Biotechnology, Division of Evolution &amp; Genomic Sciences, School of Biological Sciences, Faculty of Biology Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Zhao","sequence":"additional","affiliation":[{"name":"Manchester Fungal Infection Group, Core Technology Facility, Manchester, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Dineen","sequence":"additional","affiliation":[{"name":"Manchester Fungal Infection Group, Core Technology Facility, Manchester, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ressa","family":"Lebedinec","sequence":"additional","affiliation":[{"name":"Manchester Fungal Infection Group, Core Technology Facility, Manchester, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Bowyer","sequence":"additional","affiliation":[{"name":"Manchester Fungal Infection Group, Core Technology Facility, Manchester, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Bromley","sequence":"additional","affiliation":[{"name":"Manchester Fungal Infection Group, Core Technology Facility, Manchester, Division of Infection, Immunity and Respiratory Medicine, School of Biological Sciences, Faculty of Biology, Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Delneri","sequence":"additional","affiliation":[{"name":"Manchester Institute of Biotechnology, Division of Evolution &amp; Genomic Sciences, School of Biological Sciences, Faculty of Biology Medicine and Health University of Manchester  Manchester United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,8,20]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3004404"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt075"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.FUNK-0035-2016"},{"issue":"1","key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"pii: e01615","DOI":"10.1128\/AAC.01615-18","article-title":"Inducible cell fusion permits use of competitive fitness profiling in the human pathogenic fungus Aspergillus fumigatus","volume":"63","author":"Macdonald D.","year":"2019","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.061564.117"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780471729259.mca04as00"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0205as51"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmc.88"}],"container-title":["Current Protocols in Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.89","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpmc.89","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpmc.89","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T21:48:31Z","timestamp":1694123311000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpmc.89"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,8,20]]},"references-count":8,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1002\/cpmc.89"],"URL":"https:\/\/doi.org\/10.1002\/cpmc.89","archive":["Portico"],"relation":{},"ISSN":["1934-8525","1934-8533"],"issn-type":[{"value":"1934-8525","type":"print"},{"value":"1934-8533","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,8,20]]},"assertion":[{"value":"2019-08-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e89"}}