{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T01:50:30Z","timestamp":1790214630184,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CP Mouse Biology"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Mice are an invaluable model organism for the study of auditory function. Even though there are differences in size and frequency response, the anatomy and physiology of the mouse and human ear are remarkably similar. In addition, the tools available for genetic manipulation in the mouse have enabled the generation of models carrying mutations in orthologous human deafness\u2010causing genes, helping to validate these lesions and assess their functional consequence. Reciprocally, novel gene mutations discovered to cause auditory deficits in the mouse highlight potential new loci for human hearing loss, and expand our basic knowledge of the mechanisms and pathways important for the function of the mammalian ear. Microscopy and imaging are invaluable techniques that allow detailed characterization of cochlear pathologies associated with particular gene mutations. However, the highly organized, delicate, and intricate structures responsible for transduction of sound waves into nerve impulses are encapsulated in one of the hardest bones in the body \u2013 the temporal bone. This makes sample preparation without damage to the soft tissue, be it from dissection or processing, somewhat challenging. Fortunately, there are numerous methods for achieving high\u2010quality images of the mouse cochlea. Reported in this article are a selection of sample preparation and imaging techniques that can be used routinely to assess cochlear morphology. Several protocols are also described for immunodetection of proteins in the cochlea. In addition, the advantages and disadvantages between different imaging platforms and their suitability for different types of microscopic examination are highlighted. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmo.10","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T00:00:01Z","timestamp":1472774401000},"page":"272-306","source":"Crossref","is-referenced-by-count":8,"title":["Light and Electron Microscopy Methods for Examination of Cochlear Morphology in Mouse Models of Deafness"],"prefix":"10.1002","volume":"6","author":[{"given":"Andrew","family":"Parker","sequence":"first","affiliation":[{"name":"Mammalian Genetics Unit, MRC Harwell  Oxfordshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Chessum","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit, MRC Harwell  Oxfordshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philomena","family":"Mburu","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit, MRC Harwell  Oxfordshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Sanderson","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit, MRC Harwell  Oxfordshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Bowl","sequence":"additional","affiliation":[{"name":"Mammalian Genetics Unit, MRC Harwell  Oxfordshire United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9]]},"reference":[{"key":"e_1_2_15_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00978"},{"key":"e_1_2_15_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90343-X"},{"key":"e_1_2_15_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/002215540104901210"},{"key":"e_1_2_15_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000368399"},{"key":"e_1_2_15_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2309"},{"key":"e_1_2_15_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2014.06.006"},{"key":"e_1_2_15_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.170761"},{"key":"e_1_2_15_9_1","doi-asserted-by":"publisher","DOI":"10.1369\/0022155413478613"},{"key":"e_1_2_15_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(98)00066-1"},{"key":"e_1_2_15_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/281675a0"},{"key":"e_1_2_15_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2014.11.025"},{"key":"e_1_2_15_13_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.12-12-04575.1992"},{"key":"e_1_2_15_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1987.tb02821.x"},{"key":"e_1_2_15_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3400"},{"key":"e_1_2_15_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0020329"},{"key":"e_1_2_15_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1007061819934"},{"key":"e_1_2_15_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jnc.12243"},{"key":"e_1_2_15_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9861(19981026)400:3<299::AID-CNE1>3.0.CO;2-3"},{"key":"e_1_2_15_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/63.1.5"},{"key":"e_1_2_15_21_1","first-page":"521","article-title":"Scanning electron microscopy of the mammalian organ of Corti: Assessment of preparative procedures","volume":"6","author":"Forge A.","year":"1992","journal-title":"Scanning Microsc"},{"key":"e_1_2_15_22_1","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.072365lf"},{"key":"e_1_2_15_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2003.10.071"},{"key":"e_1_2_15_24_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016487809122718"},{"key":"e_1_2_15_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-0270(00)00322-8"},{"key":"e_1_2_15_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06091"},{"key":"e_1_2_15_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s007950070001"},{"key":"e_1_2_15_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/00016486609127043"},{"key":"e_1_2_15_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvdy.23736"},{"key":"e_1_2_15_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.201300720"},{"key":"e_1_2_15_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142956.cy0202s58"},{"key":"e_1_2_15_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heares.2008.05.009"},{"key":"e_1_2_15_33_1","first-page":"151","article-title":"[A study on the contact between tectorial membrane and inner hair cell stereocilia in the cochlea]","volume":"76","author":"Matsumura M.","year":"2001","journal-title":"Hokkaido. 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