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The percentage of absorbed cholesterol is calculated from a stable isotope\u2013based double\u2010label method. Cholesterol synthesis is calculated from MIDA after <jats:sup>13<\/jats:sup>C\u2010acetate enrichment. Cholesterol is removed from the body via the feces. The fecal excretion route is either biliary or non\u2010biliary. The non\u2010biliary route is dominated by trans\u2010intestinal cholesterol efflux, or TICE. Biliary excretion of cholesterol is measured by collecting bile. Non\u2010biliary excretion is calculated by computational modeling. In this article, we describe methods and procedures to measure and calculate dietary intake of cholesterol, fractional cholesterol absorption, fecal neutral sterol output, biliary cholesterol excretion, TICE, cholesterol synthesis, peripheral fluxes, and whole\u2010body cholesterol balance. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmo.16","type":"journal-article","created":{"date-parts":[[2016,12,14]],"date-time":"2016-12-14T20:01:43Z","timestamp":1481745703000},"page":"408-434","source":"Crossref","is-referenced-by-count":11,"title":["Measurement of Intestinal and Peripheral Cholesterol Fluxes by a Dual\u2010Tracer Balance Method"],"prefix":"10.1002","volume":"6","author":[{"given":"Onne A.H.O.","family":"Ronda","sequence":"first","affiliation":[{"name":"Center for Liver, Digestive and Metabolic Diseases, Departments of Pediatrics, University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo H.","family":"van Dijk","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Center for Liver Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.J.","family":"Verkade","sequence":"additional","affiliation":[{"name":"Center for Liver, Digestive and Metabolic Diseases, Departments of Pediatrics, University Medical Center Groningen, University of Groningen  Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert K.","family":"Groen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Center for Liver Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen  Groningen The Netherlands"},{"name":"Amsterdam Diabetes Center, Department of Vascular Medicine, Academic Medical Center  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2275(20)39690-5"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470942390.mo110118"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021576"},{"key":"e_1_2_7_5_1","article-title":"A cholesterol\u2010free, high\u2010fat diet suppresses gene expression of cholesterol transporters in murine small intestine","author":"Vogel\u2010van den Bosch H.M.","year":"2008","journal-title":"Am. 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