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For one of the principal otopathogens, non\u2010typeable <jats:italic>Haemophilus influenzae<\/jats:italic> (NTHi), animal models allow us to investigate host\u2010microbial interactions relevant to the onset and progression of infection and to study treatment of middle ear disease. We have established a robust model of NTHi middle ear infection in the <jats:italic>Junbo<\/jats:italic> mouse. Intranasal inoculation with NTHi produces high rates of bulla infection and high bacterial titers in bulla fluids; bacteria can also spread down the respiratory tract to the mouse lung. An innate immune response is detected in the bulla of <jats:italic>Junbo<\/jats:italic> mice following NTHi infection, and bacteria are maintained in some ears at least up to day 56 post\u2010inoculation. The <jats:italic>Junbo<\/jats:italic>\/NTHi infection model facilitates studies on bacterial pathogenesis and antimicrobial intervention regimens and vaccines for better treatment and prevention of NTHi middle ear infection. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpmo.24","type":"journal-article","created":{"date-parts":[[2017,3,2]],"date-time":"2017-03-02T13:12:06Z","timestamp":1488460326000},"page":"29-46","source":"Crossref","is-referenced-by-count":2,"title":["Non\u2010Typeable <i>Haemophilus influenzae<\/i> Infection of the <i>Junbo<\/i> Mouse"],"prefix":"10.1002","volume":"7","author":[{"given":"Michael T.","family":"Cheeseman","sequence":"first","affiliation":[{"name":"Developmental Biology Division, The Roslin Institute and Royal (Dick) School of Veterinary Studies University of Edinburgh  Easter Bush United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek W.","family":"Hood","sequence":"additional","affiliation":[{"name":"Molecular Genetics Unit, MRC Harwell Institute, Harwell Science and Innovation Campus  Oxfordshire United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,3,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/erv.09.63"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb1508s73"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1319051111"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1002336"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005537-200009000-00019"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-1348(02)00152-1"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1403353111"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im0106s73"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im0108s73"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142735.im0107s73"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0103s59"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-015-0420-x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01278-15"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0123154"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl403"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.021659"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3342\/ceo.2008.1.3.117"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/lary.24920"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00079-12"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302584"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00492-13"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200304000-00009"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00664-08"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmo.10"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0020149"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470942390.mo150122"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201409-1689OC"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00335-010-9295-1"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.336-363.2001"},{"key":"e_1_2_10_31_1","first-page":"749","article-title":"Acute lower respiratory tract infections in children: Possible criteria for selection of patients for antibiotic therapy and hospital admission","volume":"62","author":"Shann F.","year":"1984","journal-title":"Bull. 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