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PS2\u2010ODNs are highly stable to nucleases and numerous in vitro assays have demonstrated their biological activity. For example, PS2\u2010ODNs activated RNase H in vitro, strongly inhibited human immunodeficiency virus (HIV) reverse transcriptase, induced B\u2010cell proliferation and differentiation, and bound to protein targets in the form of PS2\u2010aptamers (thioaptamers). Thus, the interest in and promise of PS2\u2010ODNs has spawned a variety of strategies for synthesizing, isolating, and characterizing this compounds. ODN\u2010thiophosphoramidite monomers are commercially available from either AM Biotechnologies or Glen Research and this unit describes an effective methodology for solid\u2010phase synthesis, deprotection, and purification of ODNs having PS2 internucleotide linkages. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpnc.13","type":"journal-article","created":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T23:53:54Z","timestamp":1472774034000},"source":"Crossref","is-referenced-by-count":8,"title":["Solid\u2010Phase Synthesis of Oligodeoxynucleotide Analogs Containing Phosphorodithioate Linkages"],"prefix":"10.1002","volume":"66","author":[{"given":"Xianbin","family":"Yang","sequence":"first","affiliation":[{"name":"AM Biotechnologies, LLC  Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9]]},"reference":[{"key":"e_1_2_5_2_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)80801-1"},{"key":"e_1_2_5_2_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1099-1352(199703\/04)10:2<101::AID-JMR344>3.0.CO;2-4"},{"key":"e_1_2_5_2_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi960318x"},{"key":"e_1_2_5_2_4_1","doi-asserted-by":"publisher","DOI":"10.3891\/acta.chem.scand.43-0896"},{"key":"e_1_2_5_2_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/oli.1.2000.10.117"},{"key":"e_1_2_5_2_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_2_5_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0040-4039(00)88455-5"},{"key":"e_1_2_5_2_8_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.82499-0"},{"key":"e_1_2_5_2_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.03.201"},{"key":"e_1_2_5_2_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142700.nc0417s14"},{"key":"e_1_2_5_2_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac101106m"},{"key":"e_1_2_5_2_12_1","doi-asserted-by":"publisher","DOI":"10.1089\/oli.1.1996.6.133"},{"key":"e_1_2_5_2_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.7681216"},{"key":"e_1_2_5_2_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.14.6265"},{"key":"e_1_2_5_2_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0040-4039(88)85045-7"},{"key":"e_1_2_5_2_16_1","doi-asserted-by":"publisher","DOI":"10.1135\/css201112135"},{"key":"e_1_2_5_2_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.199407091"},{"key":"e_1_2_5_2_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.21.5883"},{"key":"e_1_2_5_2_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8351515"},{"key":"e_1_2_5_2_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.1.285"},{"key":"e_1_2_5_2_21_1","doi-asserted-by":"publisher","DOI":"10.1089\/ard.1994.4.269"},{"key":"e_1_2_5_2_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.2200121"},{"key":"e_1_2_5_2_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr00102a001"},{"key":"e_1_2_5_2_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/24.22.4558"},{"key":"e_1_2_5_2_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nass\/3.1.109"},{"key":"e_1_2_5_2_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2006.06.132"},{"key":"e_1_2_5_2_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo960274y"},{"key":"e_1_2_5_2_28_1","first-page":"191","article-title":"Synthesis and purification of phosphorodithioate DNA","volume":"20","author":"Wiesler W.T.","year":"1993","journal-title":"Methods Mol. 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