{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:04:33Z","timestamp":1783400673389,"version":"3.54.6"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T00:00:00Z","timestamp":1604448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["G0902250"],"award-info":[{"award-number":["G0902250"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Neuroscience"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neuronal mitochondrial fragmentation is a phenotype exhibited in models of neurodegeneration such as Parkinson's disease. Delineating the dysfunction in mitochondrial dynamics found in diseased states can aid our understanding of underlying mechanisms of disease progression and possibly identify novel therapeutic approaches. Advances in microscopy and the availability of intuitive open\u2010access software have accelerated the rate of image acquisition and analysis, respectively. These developments allow routine biology researchers to rapidly turn hypotheses into results. In this protocol, we describe the utilization of cell culture techniques, high\u2010content imaging (HCI), and the subsequent open\u2010source image analysis pipeline for the quantification of mitochondrial fragmentation in the context of a rotenone\u2010based in vitro Parkinson's disease model. \u00a9 2020 The Authors.<\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 1<\/jats:bold>\n                    : SN4741 neuron culture and treatment in a rotenone\u2010based model of Parkinson's disease\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Basic Protocol 2<\/jats:bold>\n                    : Identification of cell nuclei, measurement of mitochondrial membrane potential, and measurement of mitochondrial fragmentation in mouse\u2010derived midbrain dopaminergic neurons\n                  <\/jats:p>","DOI":"10.1002\/cpns.105","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T16:45:58Z","timestamp":1604508358000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Automated Quantification of Mitochondrial Fragmentation in an In Vitro Parkinson's Disease Model"],"prefix":"10.1002","volume":"94","author":[{"given":"Daniel J.","family":"Rees","sequence":"first","affiliation":[{"name":"Molecular Neurobiology, Institute of Life Sciences, School of Medicine Swansea University  Swansea United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Roberts","sequence":"additional","affiliation":[{"name":"Molecular Neurobiology, Institute of Life Sciences, School of Medicine Swansea University  Swansea United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Carla Carisi","sequence":"additional","affiliation":[{"name":"Molecular Neurobiology, Institute of Life Sciences, School of Medicine Swansea University  Swansea United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alwena H.","family":"Morgan","sequence":"additional","affiliation":[{"name":"Molecular Neurobiology, Institute of Life Sciences, School of Medicine Swansea University  Swansea United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Rowan","family":"Brown","sequence":"additional","affiliation":[{"name":"Centre for Nanohealth, College of Engineering Swansea University  Swansea United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey S.","family":"Davies","sequence":"additional","affiliation":[{"name":"Molecular Neurobiology, Institute of Life Sciences, School of Medicine Swansea University  Swansea United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,4]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"49\u2212103","article-title":"Live\u2010cell imaging in the study of neurodegeneration","volume":"276","author":"Bakota L.","year":"2009","journal-title":"International Review of Cell and Molecular Biology"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/81834"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.03433"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21030694"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2003.12.031"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.034223"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbi.2010.06.003"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705363105"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2005.03493.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12031-013-0075-8"},{"key":"e_1_2_6_12_1","doi-asserted-by":"crossref","unstructured":"Jones T. 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