{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:07:02Z","timestamp":1792674422461,"version":"4.5.0"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,3,22]],"date-time":"2019-03-22T00:00:00Z","timestamp":1553212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Neuroscience"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recombinant viruses are highly efficient vehicles for <jats:italic>in vivo<\/jats:italic> gene delivery. Viral vectors expand the neurobiology toolbox to include direct and rapid anterograde, retrograde, and trans\u2010synaptic delivery of tracers, sensors, and actuators to the mammalian brain. Each viral type offers unique advantages and limitations. To establish strategies for selecting a suitable viral type, this article aims to provide readers with an overview of viral recombinant technology, viral structure, tropism, and differences between serotypes and pseudotypes for three of the most commonly used vectors in neurobiology research: adeno\u2010associated viruses, retro\/lentiviruses, and glycoprotein\u2010deleted rabies viruses. \u00a9 2019 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpns.67","type":"journal-article","created":{"date-parts":[[2019,3,22]],"date-time":"2019-03-22T17:51:56Z","timestamp":1553277116000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Recombinant Viral Vectors as Neuroscience Tools"],"prefix":"10.1002","volume":"87","author":[{"given":"Shih\u2010Heng","family":"Chen","sequence":"first","affiliation":[{"name":"Viral Vector Core, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"},{"name":"Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juhee","family":"Haam","sequence":"additional","affiliation":[{"name":"Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitzie","family":"Walker","sequence":"additional","affiliation":[{"name":"Viral Vector Core, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"},{"name":"Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Scappini","sequence":"additional","affiliation":[{"name":"Signal Transduction Laboratory, Fluorescence Microscopy and Imaging Center, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Naughton","sequence":"additional","affiliation":[{"name":"Gene Transfer, Targeting and Therapeutics (GT3) Core, Salk Institute for Biological Studies  La Jolla California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Negin P.","family":"Martin","sequence":"additional","affiliation":[{"name":"Viral Vector Core, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"},{"name":"Neurobiology Laboratory, National Institute of Environmental Health Sciences, NIH\/DHHS  Research Triangle Park North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,3,22]]},"reference":[{"key":"e_1_2_9_2_1_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076310"},{"key":"e_1_2_9_2_2_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1954-08.2008"},{"key":"e_1_2_9_2_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2009.03.007"},{"key":"e_1_2_9_2_4_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0409-15.2015"},{"key":"e_1_2_9_2_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2013.189"},{"key":"e_1_2_9_2_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.166"},{"key":"e_1_2_9_2_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2005.11.015"},{"key":"e_1_2_9_2_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.4593"},{"key":"e_1_2_9_2_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41593-018-0091-7"},{"key":"e_1_2_9_2_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12354"},{"key":"e_1_2_9_2_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpmo.58"},{"key":"e_1_2_9_2_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-6606-7-17"},{"key":"e_1_2_9_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.06.014"},{"key":"e_1_2_9_2_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142301.ns0526s39"},{"key":"e_1_2_9_2_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00008-08"},{"key":"e_1_2_9_2_16_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.3863-06.2006"},{"key":"e_1_2_9_2_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3440"},{"key":"e_1_2_9_2_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23978"},{"key":"e_1_2_9_2_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2006.113050"},{"key":"e_1_2_9_2_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2013.10.016"},{"key":"e_1_2_9_2_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2707-07.2007"},{"key":"e_1_2_9_2_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142301.ns0417s57"},{"key":"e_1_2_9_2_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0702-735"},{"key":"e_1_2_9_2_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2018.01.018"},{"key":"e_1_2_9_2_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.2148"},{"key":"e_1_2_9_2_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60982-9"},{"key":"e_1_2_9_2_27_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2009.179"},{"key":"e_1_2_9_2_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2011.111"},{"key":"e_1_2_9_2_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.12.018"},{"key":"e_1_2_9_2_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3742"},{"key":"e_1_2_9_2_31_1","doi-asserted-by":"publisher","DOI":"10.1080\/23262133.2015.1122700"},{"key":"e_1_2_9_2_32_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.115253"},{"key":"e_1_2_9_2_33_1","doi-asserted-by":"publisher","DOI":"10.1089\/104303401753153947"},{"key":"e_1_2_9_2_34_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566523214666140825124311"},{"key":"e_1_2_9_2_35_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.055"},{"key":"e_1_2_9_2_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1423963112"},{"key":"e_1_2_9_2_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/10.19.2109"},{"key":"e_1_2_9_2_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-017-0234-5"},{"key":"e_1_2_9_2_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2013.094"},{"key":"e_1_2_9_2_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00649-08"},{"key":"e_1_2_9_2_41_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-0980-9_3"},{"key":"e_1_2_9_2_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.01.004"},{"key":"e_1_2_9_2_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2017.09.047"},{"key":"e_1_2_9_2_44_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.1618-13.2013"},{"key":"e_1_2_9_2_45_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120146"},{"key":"e_1_2_9_2_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.10.015"},{"key":"e_1_2_9_2_47_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142905.hg1210s54"},{"key":"e_1_2_9_2_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.14.5166"},{"key":"e_1_2_9_2_49_1","doi-asserted-by":"publisher","DOI":"10.1097\/JOM.0000000000000879"},{"key":"e_1_2_9_2_50_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001673107"},{"key":"e_1_2_9_2_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2016.08.003"},{"key":"e_1_2_9_2_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.09.021"},{"key":"e_1_2_9_2_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2009.255"},{"key":"e_1_2_9_2_54_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208592"},{"key":"e_1_2_9_2_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuron.2016.11.045"},{"key":"e_1_2_9_2_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3300938"},{"key":"e_1_2_9_3_2_1","unstructured":"https:\/\/www.addgene.org\/"},{"key":"e_1_2_9_3_4_1","unstructured":"https:\/\/www.janelia.org\/"},{"key":"e_1_2_9_3_6_1","unstructured":"http:\/\/www.gensat.org\/cre.jsp"}],"container-title":["Current Protocols in Neuroscience"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpns.67","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpns.67","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpns.67","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T21:51:51Z","timestamp":1694382711000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpns.67"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,22]]},"references-count":59,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1002\/cpns.67"],"URL":"https:\/\/doi.org\/10.1002\/cpns.67","archive":["Portico"],"relation":{},"ISSN":["1934-8584","1934-8576"],"issn-type":[{"value":"1934-8584","type":"print"},{"value":"1934-8576","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,22]]},"assertion":[{"value":"2019-03-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e67"}}