{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T12:39:04Z","timestamp":1770813544011,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2019,3,8]],"date-time":"2019-03-08T00:00:00Z","timestamp":1552003200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 1727"],"award-info":[{"award-number":["GRK 1727"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Pharmacology"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The full human skin organ culture (HSOC) model is a standardized test system for evaluating pharmacological substances on human skin <jats:italic>in vitro<\/jats:italic>. The acantholysis associated with pemphigus vulgaris (PV), a severe and potentially life\u2010threatening autoimmune skin blistering disease, can be induced in HSOC by injecting a bi\u2010specific anti\u2010desmoglein (dsg) 1 and 3 single\u2010chain antibody variable fragment (scFv). Compared to cell culture experiments (e.g., induction of dsg3\u2010internalization or keratinocyte dissociation using HaCaT cells or normal human epidermal keratinocytes) the HSOC model is more sophisticated and physiologically relevant. In this model, all three layers of the human skin are present, all cells are sustained in their physiological niche and orientation, and the cell\u2010cell\u2010contacts remain intact. Here we describe a protocol for HSOC, an <jats:italic>ex vivo<\/jats:italic> model of human skin, that has proved to be well\u2010established and informative in our laboratory. \u00a9 2019 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpph.56","type":"journal-article","created":{"date-parts":[[2019,3,8]],"date-time":"2019-03-08T14:00:07Z","timestamp":1552053607000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Translational Use of a Standardized Full Human Skin Organ Culture Model in Autoimmune Blistering Diseases"],"prefix":"10.1002","volume":"85","author":[{"given":"Imke A. K.","family":"Burmester","sequence":"first","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirin","family":"Emtenani","sequence":"additional","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan\u2010Gerrit","family":"Johns","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf J.","family":"Ludwig","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"},{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph M.","family":"Hammers","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"},{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer E.","family":"Hundt","sequence":"additional","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,3,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2018.04.008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198205203062001"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00948.x"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.291"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012615-044313"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00994"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01935"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jid.2016.07.013"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(17)30070-3"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2006.00510.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2008.339"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24185"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00692"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00858"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2011.299"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00136"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00528"},{"key":"e_1_2_7_19_1","first-page":"14387","article-title":"Experimental human cell and tissue models of pemphigus","volume":"2010","author":"Der Wier G.","year":"2010","journal-title":"Dermatology Research and Practice"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471141755.pha03es07"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471141755.pha03ds07"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1096-9896(2000)9999:9999<::AID-PATH742>3.0.CO;2-W"}],"container-title":["Current Protocols in Pharmacology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpph.56","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpph.56","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T20:26:15Z","timestamp":1694377575000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpph.56"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,8]]},"references-count":21,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,6]]}},"alternative-id":["10.1002\/cpph.56"],"URL":"https:\/\/doi.org\/10.1002\/cpph.56","archive":["Portico"],"relation":{},"ISSN":["1934-8282","1934-8290"],"issn-type":[{"value":"1934-8282","type":"print"},{"value":"1934-8290","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,8]]},"assertion":[{"value":"2019-03-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e56"}}