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Tyrosinase mutations can result in oculocutaneous albinism type 1 (OCA1), an inherited eye disease associated with decreased melanin pigment production and vision defects. To simplify expression and subsequent purification, the extracellular domain is expressed in insect cells, produced in <jats:italic>Trichoplusia ni<\/jats:italic> larvae, and purified using affinity and size\u2010exclusion chromatography. The purified recombinant human tyrosinase is a soluble monomeric glycoprotein with an activity that mirrors the tyrosinase in vivo function. \u00a9 2017 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpps.37","type":"journal-article","created":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T11:49:54Z","timestamp":1501588194000},"source":"Crossref","is-referenced-by-count":13,"title":["Purification of Recombinant Human Tyrosinase from Insect Larvae Infected with the Baculovirus Vector"],"prefix":"10.1002","volume":"89","author":[{"given":"Monika B.","family":"Dolinska","sequence":"first","affiliation":[{"name":"National Eye Institute, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul T.","family":"Wingfield","sequence":"additional","affiliation":[{"name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri V.","family":"Sergeev","sequence":"additional","affiliation":[{"name":"National Eye Institute, National Institutes of Health  Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,13]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"3.5.1","article-title":"Kinetic assay methods","volume":"5","author":"Allison R. 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