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Carbamylated proteins exert deleterious effects <jats:italic>in vivo<\/jats:italic> and are involved in the progression of various diseases, including atherosclerosis and chronic kidney disease. Therefore, there is a growing interest to evaluate the carbamylation rate of blood or tissue proteins, since carbamylation\u2010derived products (CDPs) constitute valuable biomarkers in these contexts. Homocitrulline, formed by isocyanic acid covalently attaches to the \u03b5\u2010NH<jats:sub>2<\/jats:sub> group of lysine residue side chain, is the most characteristic CDP. Sensitive and specific quantification of homocitrulline requires mass spectrometry\u2010based methods. This unit describes a liquid chromatography\u2013tandem mass spectrometry (LC\u2010MS\/MS) method for the quantification of homocitrulline, with special emphasis on pre\u2010analytical steps that allow quantification of total or protein\u2010bound homocitrulline in serum or tissue samples. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpps.56","type":"journal-article","created":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T17:54:38Z","timestamp":1523901278000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Measurement of Homocitrulline, A Carbamylation\u2010derived Product, in Serum and Tissues by LC\u2010MS\/MS"],"prefix":"10.1002","volume":"92","author":[{"given":"St\u00e9phane","family":"Jaisson","sequence":"first","affiliation":[{"name":"University of Reims Champagne\u2010Ardenne, Laboratory of Biochemistry and Molecular Biology, CNRS\/URCA UMR N\u00b07369 Extracellular Matrix 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