{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T17:22:46Z","timestamp":1790702566289,"version":"4.1.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,8,16]],"date-time":"2018-08-16T00:00:00Z","timestamp":1534377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103542"],"award-info":[{"award-number":["P20GM103542"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["currentprotocols.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["CP Protein Science"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tryptic peptide imaging is a primary workflow for matrix\u2010assisted laser desorption\/ionization imaging mass spectrometry (MALDI IMS) and has led to new information reporting highly multiplexed protein localization. Technological advances within the last few years have produced robust tools for automated spraying of both matrix and enzymes. When combined with high\u2010mass\u2010resolution and high\u2010mass\u2010accuracy instrumentation, studies now generally result in two\u2010dimensional mapping of well over 1,000 peptide peaks. This protocol describes sample preparation, spraying, and application of enzymes and matrices, and MALDI FT\u2010ICR instrumental considerations for two\u2010dimensional mapping of tryptic peptides from fresh\u2010frozen or formalin\u2010fixed, paraffin\u2010embedded tissue sections. Procedures for extraction of tryptic peptides from tissue sections for LC\u2010MS\/MS identification are also described. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpps.65","type":"journal-article","created":{"date-parts":[[2018,8,16]],"date-time":"2018-08-16T13:13:39Z","timestamp":1534425219000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh\u2010Frozen or Formalin\u2010Fixed, Paraffin\u2010Embedded Tissue"],"prefix":"10.1002","volume":"94","author":[{"given":"Peggi M.","family":"Angel","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Pharmacology &amp; Experimental Therapeutics, Medical University of South Carolina  Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Norris\u2010Caneda","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Pharmacology &amp; Experimental Therapeutics, Medical University of South Carolina  Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard R.","family":"Drake","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Pharmacology &amp; Experimental Therapeutics, Medical University of South Carolina  Charleston South Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,16]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac051534r"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2011.388"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400743a"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/2001\u20101326\u20103\u20104"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1177"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800495"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb1025s88"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsusc.2008.05.243"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2014.2788"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr401046u"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.05.007"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr3004295"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcT400003\u2010MCP200"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.5135"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2917"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2008.03.016"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac200998a"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010015\u20108532\u20106"}],"container-title":["Current Protocols in Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpps.65","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpps.65","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpps.65","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpps.65","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T05:32:12Z","timestamp":1692509532000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpps.65"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,16]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/cpps.65"],"URL":"https:\/\/doi.org\/10.1002\/cpps.65","archive":["Portico"],"relation":{},"ISSN":["1934-3655","1934-3663"],"issn-type":[{"value":"1934-3655","type":"print"},{"value":"1934-3663","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,16]]},"assertion":[{"value":"2018-08-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e65"}}