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The overall abundance of <jats:italic>S<\/jats:italic>\u2010nitrosylated proteins and reactivity of the nitrosyl group necessitates an enrichment strategy for accurate detection with adequate depth. Herein, a method is presented for the enrichment and detection of endogenous protein <jats:italic>S<\/jats:italic>\u2010nitrosylation from complex mixtures of cell or tissue lysate utilizing organomercury resin. Minimal adaptations to the method also support the detection of either <jats:italic>S<\/jats:italic>\u2010glutathionylation or <jats:italic>S<\/jats:italic>\u2010acylation using the same enrichment platform. When coupled with high accuracy mass spectrometry, these methods enable a site\u2010specific level of analysis, facilitating the curation comparable datasets of three separate cysteine post\u2010translational modifications. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpps.69","type":"journal-article","created":{"date-parts":[[2018,10,5]],"date-time":"2018-10-05T16:09:58Z","timestamp":1538755798000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Analysis of Cysteine Post Translational Modifications Using Organic Mercury Resin"],"prefix":"10.1002","volume":"94","author":[{"given":"Paschalis\u2010Thomas","family":"Doulias","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Children's Hospital of Philadelphia Research Institute  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neal S.","family":"Gould","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Children's Hospital of Philadelphia Research Institute  Philadelphia Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,10,3]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.RA117.000382"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.01.056"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12013\u2010011\u20109320\u20103"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2016.12.014"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12510"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1008036107"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2012.10.009"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2006.04.015"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2009.06.007"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2008.07.003"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R113.460261"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2015.06.010"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2014.03.007"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.04.130"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/stke.2001.86.pl1"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707723105"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u201061779\u2010885\u20106_24"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tem.2017.10.004"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.736"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aaa4312"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/AN9588300670"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2010.11.013"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M117.814327"}],"container-title":["Current Protocols in Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcpps.69","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpps.69","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cpps.69","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cpps.69","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T09:31:56Z","timestamp":1692523916000},"score":1,"resource":{"primary":{"URL":"https:\/\/currentprotocols.onlinelibrary.wiley.com\/doi\/10.1002\/cpps.69"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,3]]},"references-count":23,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1002\/cpps.69"],"URL":"https:\/\/doi.org\/10.1002\/cpps.69","archive":["Portico"],"relation":{},"ISSN":["1934-3655","1934-3663"],"issn-type":[{"type":"print","value":"1934-3655"},{"type":"electronic","value":"1934-3663"}],"subject":[],"published":{"date-parts":[[2018,10,3]]},"assertion":[{"value":"2018-10-03","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e69"}}