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Determination of protein\u2010specific <jats:italic>N<\/jats:italic>\u2010glycosylation changes in patient samples has traditionally been laborious or limited to study of a single protein per analysis. This novel technique allows for the multiplexed analysis of <jats:italic>N<\/jats:italic>\u2010glycoproteins from biofluids. Briefly, this platform consists of antibodies spotted in an array panel to a microscope slide, specific capture of glycoproteins from a biological sample, and then enzymatic release of <jats:italic>N<\/jats:italic>\u2010glycans for analysis by matrix\u2010assisted laser desorption\/ionization (MALDI) mass spectrometry (MS). <jats:italic>N<\/jats:italic>\u2010glycans are detected at each individual spot, allowing <jats:italic>N<\/jats:italic>\u2010glycan information to easily be linked back to its protein carrier. Using this protocol, multiplexed analysis of <jats:italic>N<\/jats:italic>\u2010glycosylation on serum glycoproteins can be performed. Human serum is discussed here, but this method has potential to be applied to other biofluids and to any glycoprotein that can be captured by a validated antibody. \u00a9 2019 by John Wiley &amp; Sons, Inc.<\/jats:p><jats:p><jats:bold>Basic Protocol<\/jats:bold>: Antibody panel based <jats:italic>N<\/jats:italic>\u2010glycan imaging by MALDI MS<\/jats:p><jats:p><jats:bold>Support Protocol<\/jats:bold>: Confirmation of antibody capture by IR\u2010labeled proteins<\/jats:p>","DOI":"10.1002\/cpps.99","type":"journal-article","created":{"date-parts":[[2019,11,13]],"date-time":"2019-11-13T13:19:02Z","timestamp":1573651142000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Antibody Panel Based <i>N<\/i>\u2010Glycan Imaging for <i>N<\/i>\u2010Glycoprotein Biomarker Discovery"],"prefix":"10.1002","volume":"98","author":[{"given":"Alyson P.","family":"Black","sequence":"first","affiliation":[{"name":"Department of Cell 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