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This unit describes a protocol for the culture of \u2018bronchospheres\u2019, three\u2010dimensional (3\u2010D) organoids that are derived from primary human airway basal cells. Mature bronchospheres are composed of functional multi\u2010ciliated cells, mucin\u2010producing goblet cells, and airway basal cells. In contrast to existing methods used for the culture of well\u2010differentiated human airway epithelial cells, bronchospheres do not require growth on a permeable support and can be cultured in 384\u2010well assay plates. The system provides a mechanism for investigating the regulation of basal cell fate during airway epithelial morphogenesis, as well as a basis for studying the function of the human airway epithelium in high\u2010throughput assays. \u00a9 2016 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpsc.1","type":"journal-article","created":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T20:17:41Z","timestamp":1463084261000},"source":"Crossref","is-referenced-by-count":86,"title":["Production of 3\u2010D Airway Organoids From Primary Human Airway Basal Cells and Their Use in High\u2010Throughput Screening"],"prefix":"10.1002","volume":"37","author":[{"given":"Marc","family":"Hild","sequence":"first","affiliation":[{"name":"Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research  Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aron B.","family":"Jaffe","sequence":"additional","affiliation":[{"name":"Developmental and Molecular Pathways, Novartis Institutes for BioMedical Research  Cambridge Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,5,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32526"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.12.017"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-2023(03)00032-X"},{"key":"e_1_2_7_5_1","first-page":"183","article-title":"Well\u2010differentiated human airway epithelial cell cultures","volume":"107","author":"Fulcher M.L.","year":"2005","journal-title":"Methods Mol. 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