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Traditionally, RPE lines are established from whole globes dedicated to research. Here we describe a new technique for establishing primary RPE lines from the posterior poles of globes used for corneal transplants. Since tissues from corneal transplants are derived from younger and healthier donors than those used for research, we have hypothesized that RPE cells isolated from corneal transplantation globes will result in improved primary RPE line establishment. Our new procedure increases the rate of establishing successful RPE cultures and improves the total cell number yield. Use of this advanced methodology can provide a new source of high\u2010quality primary RPE line cultures. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpsc.53","type":"journal-article","created":{"date-parts":[[2018,4,26]],"date-time":"2018-04-26T17:51:21Z","timestamp":1524765081000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Stem Cell\u2013Derived Retinal Pigment Epithelial Layer Model from Adult Human Globes Donated for Corneal Transplants"],"prefix":"10.1002","volume":"45","author":[{"given":"Marie","family":"Fernandes","sequence":"first","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai, Cell, Development, and Regenerative Biology, Black Family Stem Cell Institute  New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"McArdle","sequence":"additional","affiliation":[{"name":"The Eye\u2010Bank for Sight Restoration, Inc.  New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Schiff","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai, Cell, Development, and Regenerative Biology, Black Family Stem Cell Institute  New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy A.","family":"Blenkinsop","sequence":"additional","affiliation":[{"name":"Icahn School of Medicine at Mount Sinai, Cell, Development, and Regenerative Biology, Black Family Stem Cell Institute  New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,26]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.200810169"},{"issue":"1","key":"e_1_2_12_3_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/9780470151808.sc01c13s23","article-title":"Adaptation of stem cells to 96\u2010well plate assays: use of human embryonic and mouse neural stem cells in the MTT assay","volume":"23","author":"Behar R. 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