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For neural repair, hMSCs show translational value in research on stroke, spinal cord injury (SCI), and traumatic brain injury. It is pivotal to establish multimodal <jats:italic>in vitro<\/jats:italic> systems to investigate molecular mechanisms underlying neural actions of hMSCs. Here, we describe a platform protocol on how to set up organotypic co\u2010cultures of hMSCs (alone or polymer\u2010scaffolded) with explanted adult rat dorsal root ganglia (DRGs) to determine neural injury and recovery events for designing implants to counteract neurotrauma sequelae. We emphasize <jats:italic>in vitro<\/jats:italic> hMSC propagation, polymer scaffolding, hMSC stemness maintenance, hMSC\u2010DRG interaction profiling, and analytical formulas of neuroinflammation, trophic factor expression, DRG neurite outgrowth and tropic tracking, and <jats:italic>in vivo<\/jats:italic> verification of tailored implants in rodent models of SCI. \u00a9 2018 by John Wiley &amp; Sons, Inc.<\/jats:p>","DOI":"10.1002\/cpsc.58","type":"journal-article","created":{"date-parts":[[2018,7,19]],"date-time":"2018-07-19T07:47:07Z","timestamp":1531986427000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Establishing an Organotypic System for Investigating Multimodal Neural Repair Effects of Human Mesenchymal Stromal Stem Cells"],"prefix":"10.1002","volume":"47","author":[{"given":"Devang K.","family":"Thakor","sequence":"first","affiliation":[{"name":"Departments of Physical Medicine &amp; Rehabilitation 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