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Seventy\u2010four patients (median age 4.33, interquartile range [IQR] 2.82\u20137.23; 63.5% male) were enrolled in a prospective multicenter study: <jats:italic>in vitro<\/jats:italic> steroid inhibition of patients' peripheral blood mononuclear cell proliferation was evaluated by [methyl\u2010<jats:sup>3<\/jats:sup>H] thymidine incorporation assay at disease onset (T0) and after 4 weeks (T4) of treatment. Steroid dependence was associated with increased <jats:italic>in vitro<\/jats:italic> sensitivity at T4 assessed both as drug concentration inducing 50% of inhibition (IC<jats:sub>50<\/jats:sub>; odds ratio [OR] = 0.48, 95% confidence interval [CI] = 0.24\u20130.85; <jats:italic>P<\/jats:italic> = 0.0094) and maximum inhibition at the highest drug concentration (I<jats:sub>max<\/jats:sub>; OR = 1.13, 95% CI = 1.02\u20131.31; <jats:italic>P<\/jats:italic> = 0.017). IC<jats:sub>50<\/jats:sub> &gt; 4.4 nM and I<jats:sub>max<\/jats:sub> &lt; 92% at T4 were good predictors for optimal clinical response. These results suggest that this test may be useful for predicting the response to glucocorticoid therapy in pediatric INS.<\/jats:p>","DOI":"10.1002\/cpt.372","type":"journal-article","created":{"date-parts":[[2016,3,23]],"date-time":"2016-03-23T15:37:35Z","timestamp":1458747455000},"page":"268-274","source":"Crossref","is-referenced-by-count":6,"title":["<i>In vitro<\/i> sensitivity to methyl\u2010prednisolone is associated with clinical response in pediatric idiopathic nephrotic syndrome"],"prefix":"10.1111","volume":"100","author":[{"given":"E","family":"Cuzzoni","sequence":"first","affiliation":[{"name":"PhD, School in Sciences of Reproduction and Development University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"De Iudicibus","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Stocco","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Favretto","sequence":"additional","affiliation":[{"name":"Institute for Maternal and Child Health IRCCS Burlo Garofolo Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Pelin","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Messina","sequence":"additional","affiliation":[{"name":"Policlinico Giovanni XIII di Bari Bari Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L","family":"Ghio","sequence":"additional","affiliation":[{"name":"Pediatric Nephrology and Dialysis Unit, Department of Clinical Sciences and Community Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Monti","sequence":"additional","affiliation":[{"name":"Nephrology and Dialysis Unit, Department of Pediatrics Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Pasini","sequence":"additional","affiliation":[{"name":"Nephrology and Dialysis Unit, Department of Pediatrics Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Montini","sequence":"additional","affiliation":[{"name":"Pediatric Nephrology and Dialysis Unit, Department of Clinical Sciences and Community Health University of Milan Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Decorti","sequence":"additional","affiliation":[{"name":"Department of Life Sciences University of Trieste Trieste Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"the Nefrokid Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,6,20]]},"reference":[{"key":"e_1_2_8_1_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-014-2903-7"},{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-012-2310-x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-012-2304-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semnephrol.2009.03.015"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2013.531"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14184-0"},{"key":"e_1_2_8_7_1","first-page":"CD001533","article-title":"Corticosteroid therapy for nephrotic syndrome in children","volume":"17","author":"Hodson E.M.","year":"2007","journal-title":"Cochrane Database Syst. 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