{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:07:04Z","timestamp":1791655624974,"version":"4.3.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T00:00:00Z","timestamp":1755820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["ascpt.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin Pharma and Therapeutics"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Levofloxacin is a valuable antibiotic in the treatment of bone and joint infections. Due to the known risk of treatment failure and bacterial resistance, the development of tools facilitating pharmacokinetic\/pharmacodynamic parameter monitoring to inform precision dosing is needed. Therefore, we assessed the use of Bayesian estimation to predict AUC\n                    <jats:sub>0\u201324<\/jats:sub>\n                    and\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    of levofloxacin under various sampling scenarios realistic in clinical routine. Furthermore, we developed a free web\u2010based application allowing model\u2010informed precision dosing. All published population pharmacokinetic models of ofloxacin and levofloxacin in bone and joint infections were researched and their predictive performance was compared using a real\u2010life data cohort. We used simulated data to validate the robustness of various scenarios for Bayesian estimation of AUC\n                    <jats:sub>0\u201324<\/jats:sub>\n                    and\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    with up to three samples, including the potential impact of an incorrectly reported sampling time at peak concentration. Relative bias and relative root mean square error were estimated to assess accuracy and precision, respectively. One of the three published levofloxacin models showed negligible mean relative prediction error (0.02\u2009\u00b1\u20090.09). We modified it to a closed form approximation to facilitate the implementation in the application. The 2\u2010sample scenario (T0h\u2010T3h) allowed accurate and precise AUC\n                    <jats:sub>0\u201324<\/jats:sub>\n                    estimations for 500\u2009mg q12h and 750\u2009mg q24h dosing regimens. None of the tested scenarios allowed a satisfactory estimation of\n                    <jats:italic>C<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    . We finally developed and validated a free web\u2010based application (\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"https:\/\/levoshiny.iame-research.center\/\">https:\/\/levoshiny.iame\u2010research.center\/<\/jats:ext-link>\n                    ) using the selected model. The Shiny application will be useful in clinical practice to individualize dose regimens based on the AUC\n                    <jats:sub>0\u201324<\/jats:sub>\n                    obtained using the proposed limited sampling strategy.\n                  <\/jats:p>","DOI":"10.1002\/cpt.70044","type":"journal-article","created":{"date-parts":[[2025,8,23]],"date-time":"2025-08-23T05:10:43Z","timestamp":1755925843000},"page":"158-167","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Levofloxacin in Bone and Joint Infections: Development of an Application for Model\u2010Informed Precision Dosing"],"prefix":"10.1002","volume":"119","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3101-693X","authenticated-orcid":false,"given":"L\u00e9o","family":"Mimram","sequence":"first","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie","family":"Magreault","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"},{"name":"Unit\u00e9 Fonctionnelle de Pharmacologie, GHU Paris Seine Saint\u2010Denis, Assistance Publique\u2010H\u00f4pitaux de Paris Universit\u00e9 Sorbonne Paris Nord  Bondy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0908-3629","authenticated-orcid":false,"given":"Florian","family":"Lemaitre","sequence":"additional","affiliation":[{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) \u2013 UMR_S 1085  Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Jaureguy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Mecha\u00ef","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kam\u00e9lia","family":"Doukhi","sequence":"additional","affiliation":[{"name":"Unit\u00e9 Fonctionnelle de Pharmacologie, GHU Paris Seine Saint\u2010Denis, Assistance Publique\u2010H\u00f4pitaux de Paris Universit\u00e9 Sorbonne Paris Nord  Bondy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5971-6316","authenticated-orcid":false,"given":"Vincent","family":"Jullien","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"},{"name":"Unit\u00e9 Fonctionnelle de Pharmacologie, GHU Paris Seine Saint\u2010Denis, Assistance Publique\u2010H\u00f4pitaux de Paris Universit\u00e9 Sorbonne Paris Nord  Bondy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Comets","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"},{"name":"Univ Rennes, Inserm, EHESP, Irset (Institut de Recherche en Sant\u00e9, Environnement et Travail) \u2013 UMR_S 1085  Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6568-1041","authenticated-orcid":false,"given":"Julie","family":"Bertrand","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Cit\u00e9 and Universit\u00e9 Sorbonne Paris Nord, Inserm, IAME  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,22]]},"reference":[{"key":"e_1_2_10_1_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2020.12.010"},{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciad360"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.idnow.2023.104694"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088-199732020-00002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/383320"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.37.5.1073"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1C419"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.7.2642-2647.2005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-020-06050-1"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1345\/aph.1D337"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1086\/428053"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.279.2.125"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2022.03.041"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics9070401"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkac031"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.112053"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11040486"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v080.i03"},{"key":"e_1_2_10_19_1","unstructured":"Chang 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