{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:12:43Z","timestamp":1790820763450,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T00:00:00Z","timestamp":1514505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["UMO\u20102015\/17\/B\/NZ7\/03066"],"award-info":[{"award-number":["UMO\u20102015\/17\/B\/NZ7\/03066"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03IPT612X"],"award-info":[{"award-number":["03IPT612X"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Pharma and Therapeutics"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:p>This work revises and complements existing findings on the distribution of drug\u2010metabolizing enzymes in the first\u2010pass effect organs. We explored gene expression (quantitative polymerase chain reaction) and protein abundance (liquid chromatography\/ tandem mass spectrometry) of CYP1A2, CYP2B6, CYP2C8\/9\/19, CYP2D6, CYP2E1, CYP3A4\/5, UGT1A1\/3, UGT2B7\/15 in the liver, duodenum, jejunum, ileum, and colon in paired tissues from nine organ donors. All proteins were detected in the liver, but in the intestine CYP2C9\/19, CYP2D6, CYP3A4\/5, UGT1A1\/3, and UGT2B7 were found. CYP3A4 showed comparable abundance in the liver and jejunum, whereas other enzymes were markedly higher in the hepatic tissue. Nearly all detected enzymes showed their highest abundance in the jejunum. Significant correlations between mRNA and protein levels in liver or intestine were found for most enzymes. CYP3A4 and CYP3A5 protein abundance, but not other enzymes, were significantly correlated in the liver and the small intestine. Our data may contribute to an improved understanding of hepatic and intestinal drug metabolism.<\/jats:p>","DOI":"10.1002\/cpt.967","type":"journal-article","created":{"date-parts":[[2017,12,5]],"date-time":"2017-12-05T11:59:12Z","timestamp":1512475152000},"page":"515-524","source":"Crossref","is-referenced-by-count":131,"title":["Protein Abundance of Clinically Relevant Drug\u2010Metabolizing Enzymes in the Human Liver and Intestine: A Comparative Analysis in Paired Tissue Specimens"],"prefix":"10.1111","volume":"104","author":[{"given":"Marek","family":"Drozdzik","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Pharmacology Pomeranian Medical University Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Busch","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology University Medicine of Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Lapczuk","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Pharmacology Pomeranian Medical University Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janett","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology University Medicine of Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marek","family":"Ostrowski","sequence":"additional","affiliation":[{"name":"Department of General and Transplantation Surgery Pomeranian Medical University Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mateusz","family":"Kurzawski","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Pharmacology Pomeranian Medical University Szczecin Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Oswald","sequence":"additional","affiliation":[{"name":"Department of Clinical Pharmacology University Medicine of Greifswald Greifswald Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,29]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.2174\/0929867323666161024154457"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.105.008672"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp500330y"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/bdd.1810"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clpt.2003.10.008"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.2007.04691.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00008571-200309000-00005"},{"key":"e_1_2_9_8_1","doi-asserted-by":"crossref","first-page":"1552","DOI":"10.1016\/S0022-3565(24)37149-6","article-title":"Characterization of interintestinal and intraintestinal variations in human CYP3A\u2010dependent metabolism","volume":"283","author":"Paine M.F.","year":"1997","journal-title":"J. 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